Dynamic electrowetting on nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry.
Dynamic electrowetting on nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry.
复制标题
纳米丝硅上的动态电润湿用于无基质激光解吸/电离质谱分析。
DOI：
10.1021/ac7026029
复制
发表时间：
2008
影响因子：
7.4
通讯作者：
DeVoe,DonL
中科院分区：
文献类型：
--
作者：
Tsao,Chia-Wen;Kumar,Parshant;Liu,Jikun;DeVoe,DonL
纳米结构硅表面的动态电润湿被证明是提高无基质激光解吸/电离质谱检测灵敏度的有效方法。在没有电润湿的情况下，包含定向纳米丝致密场的硅表面显示出可以为通过疏水相互作用与纳米丝结合的沉积肽提供有效的离子生成和高光谱峰强度。通过向硅基底施加电偏压，可以通过电润湿动态控制氧化纳米丝的表面能，从而允许水性缓冲液深入渗透到纳米丝基质中。电润湿的使用被证明可以增强沉积肽和纳米丝硅表面之间的相互作用，并提高检测到的光谱峰的信噪比。有助于观察到的性能增强的一个重要特征是纳米丝表面的开孔性质，它可以防止空气被困在孔内并限制电润湿过程中的溶剂渗透。纳米丝硅和动态电润湿的组合被证明可以为一组模型肽提供几个阿托摩尔数量级的常规检测限。
Dynamic electrowetting on nanostructured silicon surfaces is demonstrated as an effective method for improving detection sensitivity in matrix-free laser desorption/ionization mass spectrometry. Without electrowetting, silicon surfaces comprising dense fields of oriented nanofilaments are shown to provide efficient ion generation and high spectral peak intensities for deposited peptides bound to the nanofilaments through hydrophobic interactions. By applying an electrical bias to the silicon substrate, the surface energy of the oxidized nanofilaments can be dynamically controlled by electrowetting, thereby allowing aqueous buffer to penetrate deep into the nanofilament matrix. The use of electrowetting is shown to result in enhanced interactions between deposited peptides and the nanofilament silicon surface, with improved signal-to-noise ratio for detected spectral peaks. An essential feature contributing to the observed performance enhancement is the open-cell nature of the nanofilament surfaces, which prevents air from becoming trapped within the pores and limiting solvent penetration during electrowetting. The combination of nanofilament silicon and dynamic electrowetting is shown to provide routine detection limits on the order of several attomoles for a panel of model peptides.