A newcomer's guide to nano-liquid-chromatography of peptides.
A newcomer's guide to nano-liquid-chromatography of peptides.
复制标题
肽纳米液相色谱新手指南
DOI：
10.1007/978-1-60761-157-8_7
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发表时间：
2009
影响因子：
--
通讯作者：
Arnold GJ
中科院分区：
文献类型：
--
作者：
Fröhlich T;Arnold GJ
LC-MS/MS是蛋白质组学领域最强大的技术之一，可以从复杂的蛋白质混合物中高通量鉴定蛋白质。除了高样品通量外，分析灵敏度是该技术的主要优点之一。灵敏的LC-MS/MS方法的先决条件是具有纳升/分钟范围内的非常低流速的色谱，通常称为纳米液相色谱（nano-LC）。然而，要执行这种分离技术，适当的仪器设置以及经验丰富的操作员是先决条件。本章的目的是帮助nano-LC新手了解这项迷人的技术。描述了纳米LC系统的技术组件，如溶剂输送系统、样品注射系统和纳米色谱柱。概述了安装、测试和操作系统的详细程序，并提供了有效故障排除的建议。
LC-MS/MS is one of the most powerful techniques in the field of proteomics allowing high throughput identification of proteins out of complex protein mixtures. Besides high sample throughput, the analytical sensitivity is one of the major benefits of this technology. A prerequisite for sensitive LC-MS/MS approaches is chromatography with very low flow rates in the nanoliter per minute range, usually referred to as nano-liquid chromatography (nano-LC). However, to perform this separation technology, an appropriate instrumental setup as well experienced operators are a prerequisite. The aim of this chapter is to help nano-LC newcomers to get introduced to this fascinating technology. Technical components of nano-LC systems like solvent delivery systems, sample injection systems, and nano-chromatography columns are described. Detailed procedures to mount, test, and operate the system are outlined, and advices for an effective troubleshooting are provided.
影响因子：
7.4
作者：
Shen, YF;Zhao, R;Smith, RD
通讯作者：
Smith, RD
开发用于耦合质谱的柱前毛细管液相色谱切换系统
DOI：
10.1002/(sici)1520-667x(1996)8:6
发表时间：
1996
期刊：
Journal of Microcolumn Separations
影响因子：
--
作者：
J. Pascual;G. Hove;A. P. Jong
通讯作者：
A. P. Jong