Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.
Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.
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与酵母 HIS4 基因座上游端粒序列插入相关的重组热点处减数分裂特异性双链 DNA 断裂的精细结构图谱。
DOI：
10.1093/genetics/143.3.1115
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Petes,TD
中科院分区：
文献类型：
--
作者：
Xu,F;Petes,TD
酿酒酵母中的减数分裂重组是由双链 DNA 断裂 (DSB) 引发的。我们使用两种方法，绘制了与通过将端粒序列插入 HIS4 上游区域而产生的重组热点相关的 DSB 的位置。我们发现断裂没有明显的序列特异性，并且位于与端粒插入相邻的约 50 bp 的区域。通过绘制断裂图谱并研究断裂末端的核酸外切酶 III 敏感性，我们得出结论，大多数断裂的 DNA 分子具有带有 3'-羟基的平末端。
Meiotic recombination inSaccharomyces cerevisiaeis initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream ofHIS4. We found that the breaks have no obvious sequence specificity and localize to a region of ~50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3′-hydroxyl groups.