Gastric pacing response evaluated with simultaneous electrical and optical mapping.
Gastric pacing response evaluated with simultaneous electrical and optical mapping.
复制标题
通过同时电和光测绘评估胃起搏反应。
DOI：
10.1109/embc48229.2022.9871138
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Cheng,LeoK
中科院分区：
文献类型：
--
作者：
Nagahawatte,NipuniD;Zhang,Hanyu;Paskaranandavadivel,Niranchan;Patton,HaleyN;Garrett,AmyS;Angeli-Gordon,TimothyR;Nisbet,Linley;Rogers,JackM;Cheng,LeoK
胃起搏是纠正异常生物电活动的一种有吸引力的治疗方法。虽然高分辨率 (HR) 电映射技术在很大程度上促进了目前对起搏对电生理功能影响的理解，但这些映射技术仅限于表面接触电极，并且信号质量可能会因起搏伪影而受到破坏。电压敏感染料的光学测绘是心脏研究中使用的另一种方法，信号质量不受起搏伪影的影响。在本研究中，我们同时应用 HR 光学和电映射技术来评估生物电慢波对胃起搏的反应。这些研究是在猪胃上进行的体内研究，其中使用高能起搏来诱导胃电活动。使用电压敏感荧光染料对起搏反应进行光学跟踪，并使用位于相邻区域的表面接触电极进行电跟踪。慢波通过光学和电学方式捕获，并且在传播时间和方向上与可比较的平均速度 () 和周期 () 一致。重要的是，光学信号没有电记录中引起的起搏伪影，这突出了光学映射的优势。临床相关性——使用光学技术对胃起搏进行牵引绘图是提高临床前对治疗理解的重大进步。因此，这些发现可以告知胃起搏治疗功能性运动障碍的功效。
Gastric pacing is an attractive therapeutic approach for correcting abnormal bioelectrical activity. While high-resolution (HR) electrical mapping techniques have largely contributed to the current understanding of the effect of pacing on the electrophysiological function, these mapping techniques are restricted to surface contact electrodes and the signal quality can be corrupted by pacing artifacts. Optical mapping of voltage sensitive dyes is an alternative approach used in cardiac research, and the signal quality is not affected by pacing artifacts. In this study, we simultaneously applied HR optical and electrical mapping techniques to evaluate the bioelectrical slow wave response to gastric pacing. The studies were conducted in vivo on porcine stomachs () where the gastric electrical activity was entrained using high-energy pacing. The pacing response was optically tracked using voltage-sensitive fluorescent dyes and electrically tracked using surface contact electrodes positioned on adjacent regions. Slow waves were captured optically and electrically and were concordant in time and direction of propagation with comparable mean velocities () and periods (). Importantly, the optical signals were free from pacing artifacts otherwise induced in electrical recordings highlighting an advantage of optical mapping. Clinical Relevance— Entrainment mapping of gastric pacing using optical techniques is a major advance for improving the preclinical understanding of the therapy. The findings can thereby inform the efficacy of gastric pacing in treating functional motility disorders.