Sensitivity of Fusarium asiaticum to a novel succinate dehydrogenase inhibitor fungicide pydiflumetofen
Sensitivity of Fusarium asiaticum to a novel succinate dehydrogenase inhibitor fungicide pydiflumetofen
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亚洲镰刀菌对新型琥珀酸脱氢酶抑制剂杀菌剂吡氟螨酯的敏感性
DOI：
10.1016/j.cropro.2017.02.011
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发表时间：
2017-06-01
期刊：
影响因子：
2.8
通讯作者：
Zhou, Ming-Guo
中科院分区：
文献类型：
--
作者：
Hou, Yi-Ping;Mao, Xue-Wei;Zhou, Ming-Guo
由亚洲镰刀菌（Fusarium asiaticum）引起的小麦赤霉病（Fusarium head blight，FHB）或黑穗病是一种毁灭性的真菌病害。在中国，多菌灵（MBC）及其与其他杀菌剂的复配制剂是防治该病害的主要杀菌剂。但田间已出现严重的抗性群体，防治该病害的杀菌剂有限，因此筛选新型杀菌剂用于防治小麦赤霉病迫在眉睫。杀菌剂氟唑菌酰羟胺（开发代号：SYN545974），即3 -（二氟甲基）- N -甲基 - N -[1 -甲基 - 2 -（2,4,6 -三氯苯基）乙基]- 1H -吡唑 - 4 -羧酰胺，是先正达公司研发的一种新型琥珀酸脱氢酶抑制剂（SDHI）。本研究建立了中国不同地区亚洲镰刀菌不同菌株对氟唑菌酰羟胺的基线敏感性。氟唑菌酰羟胺对116株亚洲镰刀菌抑制菌丝生长的半数有效浓度（EC50）值为0.019 - 0.2084μg/ml，平均值为0.0745±0.0367μg/ml。氟唑菌酰羟胺对抑制分生孢子萌发的EC50值为0.0583 - 0.4237μg/ml，平均值为0.1813±0.0861μg/ml。结果表明，该杀菌剂在体外对亚洲镰刀菌的菌丝生长和分生孢子萌发有很强的抑制作用。通过菌丝生长测定法或分生孢子萌发测定法测得的116株菌株的EC50值分布呈单峰曲线，可用于未来检测亚洲镰刀菌群体对氟唑菌酰羟胺的敏感性变化。交叉抗性分析结果表明，氟唑菌酰羟胺与多菌灵/氰烯菌酯之间没有正或负交叉抗性。在田间试验中，尽管小麦赤霉病非常严重，但2015年和2016年氟唑菌酰羟胺在125、150和200 g ai/ha时的防治效果均超过80%，显著高于氰烯菌酯在375 g ai/ha或多菌灵在600 g ai/ha时的防治效果。总之，氟唑菌酰羟胺在体外对亚洲镰刀菌有很高的活性，在田间对防治小麦赤霉病有优异的效果，还可应对对多菌灵产生抗性的亚洲镰刀菌群体。（C）2017爱思唯尔有限公司。保留所有权利。
Fusarium head blight (FHB) or scab mainly caused by Fusarium asiaticum is a devastating fungal disease in wheat. In China, carbendazim (MBC) and its co-formulated mixture with other fungicides are the main fungicides for controlling the disease. But resistant populations have developed severely in the field. The fungicides for controlling the disease are limited. So screening novel fungicides for controlling FHB is urgent. The fungicide pydiflumetofen (development code number: SYN545974),3-(Difluoromethyl)-N-methyl-N-[1-methyl-2-(2,4,6-trichlorophenyl)ethyl]-1H-pyrazole-4-carboxami.d.e, is a novel succinate dehydrogenase inhibitor (SDHI) that was developed by Syngenta. In this study, the baseline sensitivities for different isolates of F. asiaticum from various regions of China to pydiflumetofen were established. The 50% effective concentration (EC50) value of pydiflumetofen on suppressing mycelial growth against 116 isolates of F. asiaticum was 0.019-0.2084 mu g/ml and their average value was 0.0745 +/- 0.0367 mu g/ml. The EC50 value of pydiflumetofen on suppressing conidium germination was 0.0583-0.4237 mu g/ml and their average value was 0.1813 +/- 0.0861 mu g/ml. The results indicated that the fungicide had strong inhibition effect on mycelial growth and conidium germination against F asiaticum in vitro. The EC50 values distribution of 116 isolates measured by mycelial growth assay or by conidium germination assay was a unimodal curve and could be used for detecting any sensitivity changes of F. asiaticum populations to pydiflumetofen in the future. The results of cross-resistance analysis indicated that there was no positive or negative cross-resistance between pydiflumetofen and carbendazim/phenamacril. In field trials, though FHB was very severe, pydiflumetofen at 125, 150 and 200 g ai ha(-1) all provided more than 80% control efficacy which was significantly higher than that of phenamacril at 375 g ai ha(-1) or MBC at 600 g ai ha(-1) in 2015 and 2016. In conclusion, pydiflumetofen had very high activity against F. asiaticum in vitro and excellent efficacy for controlling FHB could also manage carbendazim-resistant F asiaticum populations in the field. (C) 2017 Elsevier Ltd. All rights reserved.