4-Oxo-4H-quinolizine-3-carboxylic acids as Mg2+ selective, fluorescent indicators
4-Oxo-4H-quinolizine-3-carboxylic acids as Mg2+ selective, fluorescent indicators
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DOI：
10.1021/bc000087d
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发表时间：
2001-03-01
影响因子：
4.7
通讯作者：
Levy, LA
中科院分区：
文献类型：
--
作者：
Otten, PA;London, RE;Levy, LA
本文合成了一系列新的取代4-氧代-4H-喹啉-3-羧酸，并评价了它们与镁、钙离子络合的荧光响应。这导致了第一个对镁离子具有选择性的、可比的荧光指示剂的开发。我们发现，4-氧代-4H-喹啉-3-羧酸酯是很好的荧光团，它对镁离子有很强的荧光响应，但对钙离子没有；后者金属离子经常在镁离子测量过程中与前面描述的指示剂产生干扰。被研究的荧光团的离解常数约为1.0 mM，与通常观察到的细胞内镁离子的离解常数相同。
In this paper, we present the synthesis of a new series of substituted 4-oxo-4H-quinolizine-3-carboxylic acids and the evaluation of their fluorescent response upon complexation with Mg2+ and Ca2+. This has led to the development of the first Mg2+-selective, ratioable fluorescent indicators. We found that 4-oxo-4H-quinolizine-3-carboxylates are excellent fluorophores which show a strong fluorescent response to Mg2+ but not to Ca-2; the latter metal ion often causing interference during Mg2+ measurements with previously described indicators. The dissociation constants of the studied fluorophores-around 1.0 mM-are of the same order of magnitude as is usually observed for intracellular Mg2+.