Transcription factor CncC potentially regulates the expression of multiple detoxification genes that mediate indoxacarb resistance in Spodoptera litura
Transcription factor CncC potentially regulates the expression of multiple detoxification genes that mediate indoxacarb resistance in Spodoptera litura
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DOI：
10.1111/1744-7917.12860
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发表时间：
2020-08
期刊：
影响因子：
4
通讯作者：
Li Shi;Yao Shi;Mengzhen Liu;Ya Zhang;Xiaolan Liao
中科院分区：
文献类型：
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作者：
Li Shi;Yao Shi;Mengzhen Liu;Ya Zhang;Xiaolan Liao
外源转录因子 cap ‘n’ colon isoform C (CncC) 被认为是抗氧化和解毒基因的中央调节因子。先前的研究表明，CncC 调节负责杀虫剂抗性的三相酶。在本研究中，从斜纹夜蛾中获得并鉴定了 SlituCncC 基因。定量聚合酶链反应（qPCR）分析显示，SlituCncC在所有发育阶段和组织中表达，但在3龄和4龄幼虫以及马氏小管、脂肪体和中肠中高表达。此外，与敏感菌株相比，茚虫威抗性菌株中的 SlituCncC 在茚虫威处理后上调且诱导程度更高。当暴露于茚虫威时，RNA 干扰介导的 SlituCncC 基因沉默显着增加了斜纹夜蛾的死亡率。此外，比较转录组分析显示，在 SlituCncC 敲除斜纹夜蛾中，842 个基因下调，127 个基因上调。进一步分析表明，在下调基因中鉴定出 18 种三相酶，其中 7 种与斜纹夜蛾对茚虫威抗性相关。 qPCR 分析证实这 7 个基因中有 6 个的表达与 RNA 测序数据一致。所有 6 个解毒基因均受茚虫威诱导，表达模式与 SlituCncC 相似。最后，预测了所有六个基因启动子中的 CncC-Maf 结合位点。这项研究表明转录因子 SlituCncC 可能调节介导斜纹夜蛾茚虫威抗性的多个解毒基因。
The xenobiotic transcription factor cap ‘n’ collar isoform C (CncC) is considered the central regulator of antioxidant and detoxification genes. Previous research indicated that CncC regulates three‐phase enzymes responsible for insecticide resistance. In this study, the SlituCncC gene from Spodoptera litura was obtained and characterized. Quantitative polymerase chain reaction (qPCR) analysis showed that SlituCncC was expressed in all developmental stages and tissues, but was highly expressed in 3rd‐ and 4th‐instar larvae, and in the Malpighian tubule, fat body, and midgut. In addition, SlituCncC was up‐regulated and more highly induced with indoxacarb treatment in the indoxacarb‐resistant strains compared with the susceptible strain. RNA interference‐mediated gene silencing of SlituCncC significantly increased mortality of S. litura when exposed to indoxacarb. Furthermore, comparative transcriptome analysis showed that 842 genes were down‐regulated and 127 genes were up‐regulated in SlituCncC knockdown S. litura. Further analysis indicated that 18 three‐phase enzymes were identified in the down‐regulated genes, of which seven were associated with indoxacarb resistance in S. litura. qPCR analysis confirmed that expression of six of these seven genes was consistent with RNA sequencing data. All six detoxification genes were induced by indoxacarb, and the expression patterns were similar to that of SlituCncC. Finally, the CncC–Maf binding site was predicted in all six gene promoters. This study indicates that the transcription factor SlituCncC may regulate multiple detoxification genes that mediate indoxacarb resistance in S. litura.