Observation of broken detailed balance in polymorphic transformation of bacterial flagellar filament
Observation of broken detailed balance in polymorphic transformation of bacterial flagellar filament
复制标题
细菌鞭毛丝多态性转化中细节平衡被破坏的观察
DOI：
10.1016/j.bpj.2022.05.022
复制
发表时间：
2022-06-21
影响因子：
3.4
通讯作者：
Yuan, Junhua
中科院分区：
文献类型：
--
作者：
Ma, Shuwen;Zhang, Rongjing;Yuan, Junhua
生命系统远离热力学平衡运行，这通常表现为分子尺度上的详细平衡被打破。在具有许多分子的集体功能的较大尺度下，非平衡热力学的存在可能不明显。在细菌运动中，最近发现鞭毛旋转马达的开关动力学是在非平衡状态下运行的。然而，由此产生的运动模式在中尺度，运行和翻滚的行为，通常被认为是一个泊松过程，可以描述的两个状态的平衡模型。在这里，我们研究了细节的运行和翻滚的行为，通过以下的鞭毛丝的多态性转化，观察打破详细的平衡，揭示其非平衡的性质。熵产生的评估提供了一个直接的措施，缺乏详细的平衡和量化的能量耗散率的细菌运行和翻滚调节。
Living systems operate far from thermodynamic equilibrium, which usually manifests as broken detailed balance at the molecular scale. At larger scales with collective function of many molecules, the presence of non-equilibrium thermody-namics may not be evident. In bacterial motility, the switching dynamics of the flagellar rotary motor was recently discovered to be operating in non-equilibrium. However, the resulting motility pattern at the mesoscale, the run-and-tumble behavior, was nor-mally considered to be a Poisson process that can be described by a two-state equilibrium model. Here, we studied the details of the run-and-tumble behavior by following the polymorphic transformation of the flagellar filaments, observing broken detailed balance that reveals its non-equilibrium nature. Evaluation of entropy production provided a direct measure of the lack of detailed balance and a quantification of the rate of energy dissipation for bacterial run-and-tumble regulation.