Dynamic pathology for leukocyte-platelet formation in sepsis model.
Dynamic pathology for leukocyte-platelet formation in sepsis model.
复制标题
脓毒症模型中白细胞-血小板形成的动态病理学。
DOI：
10.1016/j.jss.2014.05.016
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kusunoki M.
中科院分区：
文献类型：
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作者：
Koike Y;Tanaka K;Kobayashi M;Toiyama Y;Inoue Y;Mohri Y;Uchida K;Mizoguchi A;Kusunoki M.
本研究旨在评价脓毒症小鼠模型体内白细胞-内皮-血小板聚集的动态病理学。材料与方法采用双光子激光扫描显微镜（TPLSM）对转基因绿色荧光蛋白小鼠的脓毒症模型进行分析。通过动态血流动力学评价白细胞-内皮-血小板复合物（LEPC）形成的实时过程。结果stplsm可以在单血小板水平上直接观察LEPC的形成。与对照组相比，脓毒症急性期白细胞滚动数、滚动速度、血流速度、剪切速率随时间逐渐降低。随着时间的推移，脓毒症组的粘附白细胞数量和血小板计数逐渐增加。在脓毒症组，微循环功能障碍出现在毛细血管之前的毛细血管后静脉。结论TPLSM可实现LEPC形成的实时成像和分析，且实验间差异小。在脓毒症的急性期，新的治疗策略应该针对毛细血管后小静脉，因为它们的LEPC形成和血流动力学开始在毛细血管前发生变化。
BackgroundThis study aimed to evaluate the dynamic pathology forin vivoreal-time leukocyte–endothelium–platelet aggregation in a mouse model of sepsis.Materials and methodsA lipopolysaccharide-induced model of sepsis was analyzed in green fluorescent protein transgenic mice using two-photon laser-scanning microscopy (TPLSM). The real-time process of leukocyte–endothelium–platelet complex (LEPC) formation was assessedin vivousing blood flow dynamics.ResultsTPLSM allowed direct visualization of LEPC formation at the single-platelet level. Leukocytes rolling number and speed, blood flow velocity, and shear rate gradually decreased with time during the acute phase of sepsis compared with those in the control groups. The number of adherent leukocytes and platelet counts gradually increased over time in the septic group. In the septic group, microcirculatory dysfunction was seen in the postcapillary venules before the capillaries.ConclusionsIn vivoreal-time imaging and analysis of LEPC formation can be achieved with little inter-experimental variation using TPLSM. In the acute phase of sepsis, new treatment strategies should target the postcapillary venules because their LEPC formation and blood flow dynamics start to change before those in the capillaries.