Ultrastructural changes in hepatocytes of precision-cut rat liver slices after incubation for 24 and 48 hours
Ultrastructural changes in hepatocytes of precision-cut rat liver slices after incubation for 24 and 48 hours
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DOI：
10.1078/0940-2993-00292
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发表时间：
2003-06-01
影响因子：
--
通讯作者：
Müller, D
中科院分区：
文献类型：
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作者：
Neupert, D;Glöckner, R;Müller, D
通过透射电子显微镜研究了长期孵育（24-48 h）后与新鲜制备的切片相比，精密切割的大鼠肝切片的肝细胞，表明细胞的可逆和不可逆的细胞内变化。孵育24小时后，切片横切面的形态学图像特征为受损和坏死细胞的中心区域，两侧为两至几个活细胞的表面层。这是典型的氧张力和营养物含量从切片表面到中心的扩散梯度。在适应细胞的表面上的切片，我们观察到一个无细胞器层的细颗粒物质在顶端的细胞质，其次是平行取向的堆栈附近的粗面内质网。线粒体的基本上正常的外观在适应细胞不包含絮状密度，这是观察到的受损细胞只。实质细胞的细胞质由明确区域的透明细胞质物质组成，其中含有光滑内质网的许多分支管状轮廓，推测在糖原聚集体耗尽的区域。坏死的亚细胞体征是线粒体破坏，内质网扩张，无核糖体，染色质在肝细胞核内聚集。在孵育24和48 h之间观察到细胞器无明显差异，但坏死迹象的发生率和强度随孵育时间和切片厚度的增加而增加。这些变化的过程可能反映了细胞适应的现象和缺氧细胞损伤的边缘和中心的切片，分别。
Hepatocytes of precision-cut rat liver slices were studied by means of transmission electron microscopy after long-term incubation (24-48 h) in comparison with freshly prepared slices, indicating reversible and irreversible intracellular alterations of the cells. After 24 h incubation the morphological image in transversal sections of slices is characterised by a central zone of damaged and necrotic cells flanked by two to several superficial layers of viable cells. This is typical of a diffusion gradient of oxygen tension and nutrient content from the surface to the centre of the slices. In adapted cells on the surface of the slices we observed an organelle-free layer of fine granular material in the apical cytoplasm followed by parallel oriented stacks of rough endoplasmic reticulum near by. Mitochondria of essentially normal appearance in adapted cells did not contain flocculent densities, which were observed in damaged cells only. The cytoplasm of parenchymal cells consisted of defined areas of clear cytoplasmic material containing numerous branching tubular profiles of smooth endoplasmic reticulum, presumably in the regions with depleted glycogen aggregates. Subcellular signs of necrosis are destroyed mitochondria, dilated endoplasmic reticulum free of ribosomes and clumping of chromatin in the nucleus of hepatocytes. No appreciable differences of the cell organelles were observed between 24 and 48 h of incubation, but the incidence and intensity of signs of necrosis increased with the duration of incubation and the thickness of the slices. The process of these changes may reflect the phenomenon of cellular adaptation and of hypoxic cellular injury in the periphery and the centre of the slices, respectively.