Acid-induced unfolding of myoglobin triggered by a laser pH jump method
Acid-induced unfolding of myoglobin triggered by a laser pH jump method
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DOI：
10.1039/b516533d
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发表时间：
2006-01-01
影响因子：
3.1
通讯作者：
Corrie, John E. T.
中科院分区：
文献类型：
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作者：
Abbruzzetti, Stefania;Sottini, Silvia;Corrie, John E. T.
以1-（2-硝基苯基）乙基硫酸盐（笼状硫酸盐）为光活化笼状质子，我们可以用一个纳秒激光脉冲诱导肌红蛋白的完全酸解折叠。这是可能的，因为光不稳定化合物释放的质子浓度高（接近mM）。该化合物产生大的pH跳变的能力增加，因为其他光产物（2-亚硝基苯乙酮和硫酸根离子）不缓冲释放的质子。展开动力学的完整时间过程，跨越从毫秒到几秒的范围内，可以通过监测在633 nm处的可见光谱中的吸光度变化来准确地再现。
Using 1-(2-nitrophenyl)ethyl sulfate (caged sulfate) as a photoactivatable caged proton, we could induce complete acid unfolding of myoglobin with a single nanosecond laser pulse. This was possible because of the high (approximate to mM) concentration of protons released by the photolabile compound. The ability of the compound to produce a large pH jump arises because the other photoproducts (2-nitrosoacetophenone and sulfate ion) do not buffer the released protons. The complete time course of the unfolding kinetics, spanning a range from milliseconds to several seconds, could be accurately reproduced by monitoring absorbance changes in the visible spectrum at 633 nm.