Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha
Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha
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DOI：
10.1089/mab.2020.0002
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发表时间：
2020-08-01
影响因子：
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通讯作者：
Mizuno, Takuya
中科院分区：
文献类型：
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作者：
Sakai, Osamu;li, Tatsuhito;Mizuno, Takuya
对癌症微环境的了解在癌症研究中起着至关重要的作用。需要一种工具来评估癌细胞周围的免疫细胞。本研究建立并评价了一种新的抗犬CD8α的单抗(cCD8α)。用表达cCD8α的细胞免疫大鼠产生抗体。在杂交瘤细胞的建立和筛选后，建立了F3-B2克隆。用cCD8α过表达的小鼠细胞证实了F3-B2的反应性。流式细胞仪分析还表明，F3-B2与犬外周血单核细胞和犬T细胞淋巴瘤细胞系中自然表达的cCD8α发生反应。淋巴组织标本F3-B2免疫组织化学染色。此外，我们还发现F3-B2具有与猫科动物CD8的反应性。因此，该抗体为分析犬和猫肿瘤中CD8阳性的细胞毒性淋巴细胞提供了一个很好的研究工具。
Understanding of the microenvironment of cancer plays a crucial role in cancer research. A tool is needed to evaluate the immune cells surrounding the cancer cells. This study establishes and evaluates a novel monoclonal antibody against canine CD8 alpha (cCD8 alpha). The antibody was produced by immunization of rats with cCD8 alpha-expressing cells. After establishment and selection of hybridoma cells, the clone F3-B2 was established. The reactivity of F3-B2 was confirmed using cCD8 alpha-overexpressing murine cells. Flow cytometric analysis also demonstrated that F3-B2 reacts with cCD8 alpha naturally expressed in canine peripheral blood mononuclear cells and a canine T cell lymphoma cell line. The specimens of lymphoid tissue showed immunohistochemical staining for F3-B2. Moreover, we also found that F3-B2 exhibited reactivity against feline CD8. Thus, this antibody provides a good research tool to analyze CD8-positive cytotoxic lymphocytes in canine and feline tumors.