Sequence analysis of the internal transcribed spacer 2 (ITS2) from Philornis seguyi (García, 1952) and Philornis torquans (Nielsen, 1913) (Diptera: Muscidae)
Sequence analysis of the internal transcribed spacer 2 (ITS2) from Philornis seguyi (García, 1952) and Philornis torquans (Nielsen, 1913) (Diptera: Muscidae)
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DOI：
10.1007/s11230-013-9428-5
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发表时间：
2013-08
影响因子：
1.3
通讯作者：
L. Monje;M. Quiroga;D. Manzoli;M. Couri;L. Silvestri;J. Venzal;P. Cuervo;P. Beldomenico
中科院分区：
文献类型：
--
作者：
L. Monje;M. Quiroga;D. Manzoli;M. Couri;L. Silvestri;J. Venzal;P. Cuervo;P. Beldomenico
PhilornisMeinert，1890（双翅目：家蝇科）是新热带区双翅目的一个寄生于鸟类的属。目前使用的外部形态特征来区分本属内的种存在一定的局限性。我们利用rRNA基因的第二个内转录间隔区（ITS 2）作为分子标记，对来自不同地区的Philornis torquan和Philornis seguyi的成虫标本进行了区分。标本鉴定为P.来自马格达莱纳（布宜诺斯艾利斯省）的seguyi显示ITS2序列不同于P. torquans，而所有其他标本的P. segui的序列与P的序列相同。扭矩这些发现并不一定证实来自马格达莱纳的标本确实属于P。seguyi，nor that P. seguyi是一个有效的物种。相反，他们提醒我们，当单独使用形态特征时，物种可能会被错误识别。利用分子生物学方法辅助鉴定嗜蚜蝇属。torquansis在门多萨省和乌拉圭首次报道。
PhilornisMeinert, 1890 (Diptera: Muscidae) is a genus of Neotropical dipterans that parasitise birds. The currently used external morphological characters to distinguish between species within this genus present some limitations. We used the second internal transcribed spacer region (ITS2) of the rRNA gene as a molecular marker to differentiate adult specimens ofPhilornisidentified morphologically asPhilornis torquansandPhilornis seguyifrom different localities. Specimens identified asP. seguyifrom Magdalena (Buenos Aires Province) showed an ITS2 sequence different from that forP. torquans, whereas all other specimens ofP. seguyihad sequences identical to those forP. torquans. These findings do not necessarily confirm that specimens from Magdalena indeed belong toP. seguyi, nor thatP. seguyiis a valid species. Instead, they alert us about the potential for species misidentification when using morphological characters alone. The use of molecular approaches to aid the identification ofPhilornisspp. will shed light on the systematics of this group.P. torquansis reported for the first time in Mendoza Province and Uruguay.