Protein corona over silver nanoparticles triggers conformational change of proteins and drop in bactericidal potential of nanoparticles: Polyethylene glycol capping as preventive strategy
Protein corona over silver nanoparticles triggers conformational change of proteins and drop in bactericidal potential of nanoparticles: Polyethylene glycol capping as preventive strategy
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DOI：
10.1016/j.colsurfb.2016.06.050
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发表时间：
2016-10-01
影响因子：
5.8
通讯作者：
Paul, Subhankar
中科院分区：
文献类型：
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作者：
Ban, Deependra Kumar;Paul, Subhankar
在这里，我们证明，淀粉封端的银纳米粒子（AgNPST）的大小范围为10-15 nm，可以很容易地与小蛋白牛α-乳白蛋白（BLA）通过蛋白质冠的形成相互作用。我们进一步观察到，这种现象不仅导致BLA的结构变化，但在AgNP的杀菌潜力急剧下降。为了设计一种策略，以尽量减少蛋白质吸附和最大限度地保留杀菌潜力的AgNP，我们开发了稳定的聚乙二醇（PEG）封端的AgNP（AgNPPEG），清楚地表明减少蛋白质的构象变化和保留大量的杀菌潜力的AgNPPEG，相比AgNPST。此外，AgNPPEG还显示出良好的血液相容性。一个相对较大的蛋白质牛血清白蛋白（BSA）和人血清溶液中含有血清蛋白也被用于本研究中，以验证我们的假设。总的来说，我们的研究确定，蛋白质包被的AgNP基本上失去了其固有的杀菌潜力;然而，当用合适的材料如PEG功能化时，它可以大量减少这种下降。此外，它在实际生理条件下实现了改善的生物相容性，可能在许多细菌介导的疾病中找到更好的治疗途径。(C)© 2016 Elsevier B. V.版权所有。
Here, we demonstrated that starch-capped silver nanoparticles (AgNPST) with a size range of 10-15 nm could readily interact with a small protein bovine alpha-lactalbumin (BLA) through the formation of protein corona. We further observed that such phenomena not only caused structural change of BLA but drastic drop in the bactericidal potential of AgNP. To design a strategy towards minimizing protein adsorption and maximizing the retention of bactericidal potential of AgNP, we developed stable polyethylene glycol (PEG)-capped AgNP (AgNPPEG) that clearly demonstrated reduced conformational changes of protein and retention of substantial bactericidal potential of AgNPPEG, compared to AgNPST. Moreover, AgNPPEG also showed excellent hemocompatibility. A relatively larger protein bovine serum albumin (BSA) and human blood serum solution containing serum proteins were also used in this study to validate our hypotheses. Overall, our study established that protein coated AgNP losses its inherent bactericidal potential substantially; however, when functionalized with a suitable material such as PEG, it could reduce such drop in substantial amount. Moreover, it achieved improved biocompatibility in actual physiological condition that might find a better therapeutic avenue in many bacteria-mediated disorders. (C) 2016 Elsevier B.V. All rights reserved.