Identification of downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate signal pathway in pulmonary hypertension.
Identification of downstream target genes regulated by the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate signal pathway in pulmonary hypertension.
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一氧化氮可溶性鸟苷酸环化酶-环磷酸鸟苷信号通路调控肺动脉高压下游靶基因的鉴定
DOI：
10.1177/0300060516636751
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Zou L;Xu X;Zhai Z;Yang T;Jin J;Xiao F;Wang C
探讨一氧化氮-可溶性鸟苷酸环化酶-环鸟苷单磷酸（NO-sGC-cGMP）信号通路调控的下游靶基因及其在肺动脉高压（PH）发病机制中的可能作用。使用 8-溴环单磷酸鸟苷、BAY 41-2272 和 BAY 60-2770 进行数字基因表达标签分析，以鉴定在人肺动脉平滑肌细胞中激活 NO-sGC-cGMP 信号通路后差异表达的基因。结果通过实时聚合酶链式反应得到证实。还进行了基因本体和信号转导网络分析。许多基因存在差异表达，包括 MMP1、SERPINB2、GREM1 和 IL8。总共发现了 68 个基因本体术语和 7 个通路与这些基因相关。这些基因大多数参与细胞增殖、细胞迁移和凋亡，可能有助于PH的病理性肺血管重塑。这些结果可能为PH的分子机制提供新的见解。
To investigate the downstream target genes regulated by the nitric oxide–soluble guanylate cyclase–cyclic guanosine monophosphate (NO-sGC-cGMP) signal pathway and their possible roles in the pathogenesis of pulmonary hypertension (PH). Digital gene expression tag profiling was performed to identify genes that are differentially expressed after activation of the NO-sGC-cGMP signal pathway in human pulmonary artery smooth muscles cells using 8-bromo-cyclic guanosine monophosphate, BAY 41-2272 and BAY 60-2770. Results were confirmed using real-time polymerase chain reaction. Gene ontology and signal transduction network analyses were also performed. A number of genes were differentially expressed, including MMP1, SERPINB2, GREM1 and IL8. A total of 68 gene ontology terms and seven pathways were found to be associated with these genes. Most of these genes are involved in cell proliferation, cell migration and apoptosis, which may contribute to the pathological pulmonary vascular remodelling in PH. These results may provide new insights into the molecular mechanisms of PH.
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影响因子：
3.7
作者：
Jacquin S;Rincheval V;Mignotte B;Richard S;Humbert M;Mercier O;Londoño-Vallejo A;Fadel E;Eddahibi S
通讯作者：
Eddahibi S
影响因子：
120.7
作者：
Gaine, S
通讯作者：
Gaine, S
影响因子：
158.5
作者：
GIAID, A;YANAGISAWA, M;STEWART, DJ
通讯作者：
STEWART, DJ
水通道蛋白 1 通过 p38-MAPK 途径参与肝肺综合征大鼠血清诱导的肺动脉平滑肌细胞迁移。
影响因子：
--
作者：
Gao, Jing;Chen, Lin;Yi, Bin
通讯作者：
Yi, Bin
DOI：
10.1016/j.biocel.2008.01.028
发表时间：
2008-01-01
影响因子：
4
作者：
Kouri, Fotini M.;Queisser, Markus A.;Eickelberg, Oliver
通讯作者：
Eickelberg, Oliver