Genetic profile and serosurvey for virus infections of Japanese wild boars in Shikoku Island

Genetic profile and serosurvey for virus infections of Japanese wild boars in Shikoku Island
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DOI:
10.1292/jvms.67.563
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发表时间:
2005-06-01
影响因子:
1.2
通讯作者:
Nishimura, M
Nishimura, M
中科院分区:
农林科学4区
文献类型:
--
作者:
Ishiguro, N;Nishimura, M

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为了解日本野猪对四国县(香川县、德岛县、Kouchi县和爱原县)的抑制遗传背景,对189头野猪的线粒体DNA (mtDNA)单倍型和核糖磷酸异构酶加工伪基因(GPIP)基因型进行了检测。在四国岛检测到的8种不同mtDNA单倍型(J5、J10、J12、J13、J15、J19、J20和E33)中,首先鉴定出的是日本野猪系的J19和J20,以及欧洲家猪系的E33。E33单倍型的存在表明野猪与出逃家猪和/或杂交的Inobuta进行了本地杂交。在四国岛189只野公猪中检测到4种gip基因型,但未发现欧洲gip等位基因。在113只野猪中,没有检测到抗经典猪瘟或奥杰斯基病的抗体,这表明它们没有接触过这些病原体。
To estimate the genetic background of Japanese wild boars inhibiting the 4 prefectures of Shikoku Island (Kagawa, Tokushima, Kouchi and Ehime), we examined haplotypes of mitochondrial DNA (mtDNA) and genotypes of the nuclear glucosephosphate isomerase-processed pseudogene (GPIP) in 189 wild boars. Of the 8 different mtDNA haplotypes (J5, J10, J12, J13, J15, J19, J20 and E33) detected in Shikoku Island, the first to be identified were J19 and J20 (of Japanese wild boar lineage) and E33 (of European domestic pig lineage). The presence of haplotype E33 indicates local crossbreeding between wild boar and escaped domestic pigs and/or crossbred Inobuta. Four kinds of the GPIP genotypes were detected from 189 wild baors in Shikoku Island, but no European GPIP alleles were found. In 113 wild boars, no antibodies against Classical Swine Fever or Aujeszky's disease were detected, suggesting that they had not been exposed to those pathogens.