MicroRNA-184 promotes apoptosis of trophoblast cells via targeting WIG1 and induces early spontaneous abortion.
MicroRNA-184 promotes apoptosis of trophoblast cells via targeting WIG1 and induces early spontaneous abortion.
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MicroRNA-184通过靶向WIG1促进滋养层细胞凋亡并诱导早期自然流产。
DOI：
10.1038/s41419-019-1443-2
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发表时间：
2019
影响因子：
9
通讯作者：
Jin Li ping
中科院分区：
文献类型：
--
作者：
Zhang Yuan;Zhou Ji;Li Ming qing;Xu Jie;Zhang Jin ping;Jin Li ping
复发性自然流产（RSA）是指严重威胁人类生殖健康的连续两次或两次以上妊娠的意外终止。我们之前的研究表明，miR-184在RSA中的表达高于正常妊娠，无论是在绒毛还是蜕膜中。在这项研究中，与正常孕妇相比，RSA患者的蜕膜基质细胞（DSC）和蜕膜免疫细胞（DIC）以及外周血中miR-184的表达同样增强。此外，我们还发现miR-184可以促进滋养层细胞的凋亡，抑制滋养层细胞的增殖。进一步的研究表明，miR-184通过靶向WIG 1上调Fas的表达，从而诱导细胞凋亡。最后，体内过表达miR-184后，孕鼠的胚胎吸收率显著增加。因此，我们的研究概述了miR-184在维持成功妊娠中的关键作用，为RSA提供了新的诊断和治疗靶点。
Recurrent spontaneous abortion (RSA) refers to the unintentional termination of two or more consecutive pregnancies that severely threatens human reproductive health. Our previous study has shown that miR-184 is expressed more highly in RSA than in normal pregnancy, whether in the villus or decidua. In this study, compared with normal pregnant women, the expression of miR-184 in decidual stromal cells (DSCs) and decidual immune cells (DICs), as well as in peripheral blood, from RSA patients was enhanced similarly. Moreover, we found miR-184 could promote the apoptosis and repress the proliferation of trophoblast cells. Further exploration indicated that miR-184 upregulated the expression of Fas by targeting WIG1 thus inducing cell apoptosis. Finally, after miR-184 overexpression in vivo, the embryo resorption rate in pregnant mice was increased significantly. Therefore, our study outlines the pivotal role of miR-184 in maintaining successful pregnancy, providing a new diagnostic and therapeutic target for RSA.