The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice
The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice
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DOI：
10.1016/j.bbrc.2017.10.128
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Jiang, Mingyi
中科院分区：
文献类型：
--
作者：
Huang, Liping;Jia, Jing;Jiang, Mingyi
种子萌发是一个重要的发育过程。脱落酸（阿坝）是种子萌发的重要抑制因子，而活性氧（ROS）在种子萌发过程中也起着重要的调控作用。阿坝能抑制种子萌发过程中活性氧的产生，但阿坝抑制种子萌发过程中活性氧产生的机制尚不清楚。通过ChIP（chromatin immunoprecipitation）测序，我们发现水稻锌指转录因子ZFP 36可以直接与OsAPX 1启动子结合，编码一种抗坏血酸过氧化物酶（APX），该酶对H2O2（ROS的一种底物）的亲和力最强，是OsAPX 1启动子的转录激活因子。此外，ZFP 36可以与胚胎发生后期丰富的蛋白OsLEA5相互作用，共同调节OsAPX 1的启动子活性。阿坝处理对ZFP 36过表达植株的种子萌发有明显的抑制作用，而OsLEA5的RNA干扰（RNAi）突变体对阿坝的敏感性较低，外源ASC（抗坏血酸）可以缓解阿坝对种子萌发的抑制。因此，OsAPX1是ZFP36的直接作用靶点，OsLEA5可能通过调控OsAPX1的表达，与ZFP36相互作用，共同调控ABA抑制的种子萌发。(C)2017由Elsevier Inc.出版
Seed germination is a vital developmental process. Abscisic acid (ABA) is an essential repressor of seed germination, while ROS (reactive oxygen species) also plays a vital role in regulating seed germination. ABA could inhibit the production of ROS in seed germination, but the mechanism of ABA reduced ROS production in seed germination was hitherto unknown. Here, by ChIP (chromatin immunoprecipitation)seq, we found that ZFP36, a rice zinc finger transcription factor, could directly bind to the promoter of OsAPX1, coding an ascorbate peroxidase (APX) which has the most affinity for H2O2 (substrate; a type of ROS), and act as a transcriptional activator of OsAPX1 promoter. Moreover, ZFP36 could interact with a late embryogenesis abundant protein OsLEA5 to co-regulate the promoter activity of OsAPX1. The seed germination is highly inhibited in ZFP36 overexpression plants under ABA treatment, while an RNA interference (RNAi) mutant of OsLEA5 rice seeds were less sensitive to ABA, and exogenous ASC (ascorbate acid) could alleviate the inhibition induced by ABA. Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1. (C) 2017 Published by Elsevier Inc.