Direct evidence of active and rapid nuclear degradation triggered by vacuole rupture during programmed cell death in Zinnia
Direct evidence of active and rapid nuclear degradation triggered by vacuole rupture during programmed cell death in Zinnia
复制标题
DOI：
10.1104/pp.125.2.615
复制
发表时间：
2001-02-01
期刊：
影响因子：
7.4
通讯作者：
Fukuda, H
中科院分区：
文献类型：
--
作者：
Obara, K;Kuriyama, H;Fukuda, H
在维管植物的发育过程中，分化为导管分子（TE）是程序性细胞死亡（PCD）的一个典型例子。在PCD过程中，TE降解其细胞内容物，并成为充当水导管的中空尸体。使用百日菊（百日菊）细胞培养，我们获得了一系列的观察单个活细胞进行TE PCD的共聚焦激光扫描显微镜。进行活体染色并测量相对荧光强度，揭示TE中肿胀液泡的液泡膜在其物理破裂之前失去荧光素的选择性渗透性。空泡破裂后，细胞核在10 ~ 20 min内迅速降解，但未出现明显的染色质浓缩或核碎裂现象。叶绿体中的类核也以与细胞核相似的时间进程降解。降解没有发生在非TE被迫打破的液泡丙磺舒治疗。这些结果表明，TE分化涉及一种独特类型的PCD，其中活性和快速的核降解是由液泡破裂引发的。
Differentiation into a tracheary element (TE) is a typical example of programmed cell death (PCD) in the developmental processes of vascular plants. In the PCD process the TE degrades its cellular contents and becomes a hollow corpse that serves as a water conduct. Using a zinnia (Zinnia elegans) cell culture we obtained serial observations of single living cells undergoing TE PCD by confocal laser scanning microscopy. Vital staining was performed and the relative fluorescence intensity was measured, revealing that the tonoplast of the swollen vacuole in TEs loses selective permeability of fluorescein just before its physical rupture. After the vacuole ruptured the nucleus was degraded rapidly within 10 to 20 min. No prominent chromatin condensation or nuclear fragmentation occurred in this process. Nucleoids in chloroplasts were also degraded in a similar time course to that of the nucleus. Degradations did not occur in non-TEs forced to rupture the vacuole by probenecid treatment. These results demonstrate that TE differentiation involves a unique type of PCD in which active and rapid nuclear degradation is triggered by vacuole rupture.