WHEAT HIGH-TEMPERATURE TOLERANCE DURING REPRODUCTIVE GROWTH .1. EVALUATION BY CHLOROPHYLL FLUORESCENCE
WHEAT HIGH-TEMPERATURE TOLERANCE DURING REPRODUCTIVE GROWTH .1. EVALUATION BY CHLOROPHYLL FLUORESCENCE
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DOI：
10.2135/cropsci1990.0011183x003000040024x
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发表时间：
1990-07-01
期刊：
影响因子：
2.3
通讯作者：
PAULSEN, GM
中科院分区：
文献类型：
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作者：
MOFFATT, JM;SEARS, RG;PAULSEN, GM
光合作用对高温胁迫高度敏感，这是许多地区小麦（Triticum aestivum L.）生殖生长过程中的一个重要问题。我们的目的是评估叶绿素荧光，它间接测量光合作用效率，作为小麦耐高温性的筛选标准。在两个受控环境和田间测试了对南部大平原适应能力不同的六种基因型。受控环境中的植物在籽粒灌浆期间和从孕穗后期到花后10天的不同发育阶段经受37/25℃和25/20℃日/夜条件。田间试验在冬末进行，以增加花后高温胁迫的可能性。使用便携式荧光计在完整植物的旗叶上测量初始（Fo）、变量（Fv）和最大荧光（Fm）。在受控环境和现场条件下，基因型之间的荧光参数存在显着差异。花后对所有基因型进行高温处理时，Fv的相对稳定性与产量呈正相关。 Fv 和谷物产量在受控环境中呈负相关，但在田间呈正相关且不显着相关。高Fv组的基因型也属于高产组。叶绿素荧光，尤其是 Fv，可能是筛选相似成熟度的小麦在生殖生长过程中耐高温能力的有用工具，但该技术应与其他可能表明抗逆性的标准一起使用。
Photosynthesis is highly sensitive to high temperature stress, an important problem in many regions during reproductive growth of wheat (Triticum aestivum L.). Our objective was to evaluate chlorophyll flourescence, which indirectly measures photosynthetic efficiency, as a screening criterion for high temperature tolerance in wheat. Six genotypes differing in adapatation to the southern Great Plains were tested in two controlled environments and in the field. Plants in controlled environments were subjected to 37/25.degree.C and 25/20.degree.C day/night regimes during grain filling and at different developmental stages from late boot to 10 d after anthesis. The field experiment was planted during late winter to increase the probability of high temperature stress after anthesis. Initial (Fo), variable (Fv), and maximum flourescence (Fm) were measured using a portable fluorometer on flag leaves of intact plants. Fluorescence parameters differed significantly among genotypes in controlled environments and field conditions. Relative stability of Fv was positively related to yield when high temperature treatment was applied to all genotypes after anthesiss. The Fv and grain yield were negatively correlated in controlled environments but positively and not significantly correlated in the field. Genotypes in the high Fv group were also in the high yield group. Chlorphyll flourescence, especially Fv, may be a useful tool for screening wheat of similar maturity for high temperature tolerance during reproductive growth, but the technique should be used with other criteria that might indicate resistance to stress.