Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells.
Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells.
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DOI：
10.1016/j.gdata.2014.04.002
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发表时间：
2014-12-01
期刊：
影响因子：
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通讯作者：
Ding, Han-Fei
中科院分区：
文献类型：
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作者：
Wang, Xiangwei;Yang, Liqun;Choi, Jeong-Hyeon;Kitamura, Eiko;Chang, Chang-Sheng;Ding, Jane;Lee, Eun J;Cui, Hongjuan;Ding, Han-Fei
诱导分化是神经母细胞瘤的一种治疗策略，神经母细胞瘤是一种常见的儿童交感神经系统癌症。同源框蛋白HOXC9是神经母细胞瘤分化的关键调节因子。为了从分子水平上了解HOXC9在促进神经母细胞瘤细胞分化中的作用，我们通过基因表达谱芯片和染色质免疫沉淀结合大规模平行测序(CHIP-SEQ)对HOXC9诱导的分化程序进行了全基因组分析。在这里，我们详细描述了产生微阵列和芯片序列数据的实验系统、方法和质量控制，这些数据与我们最近发表的文献[1]有关。
Induction of differentiation is a therapeutic strategy in neuroblastoma, a common pediatric cancer of the sympathetic nervous system. The homeobox protein HOXC9 is a key regulator of neuroblastoma differentiation. To gain a molecular understanding of the function of HOXC9 in promoting differentiation of neuroblastoma cells, we conducted a genome-wide analysis of the HOXC9-induced differentiation program by microarray gene expression profiling and chromatin immunoprecipitation in combination with massively parallel sequencing (ChIP-seq). Here we describe in detail the experimental system, methods, and quality control for the generation of the microarray and ChIP-seq data associated with our recent publication [1].