Semiquantitative detection of cytokine messages in X-irradiated and regenerating rat thymus
Semiquantitative detection of cytokine messages in X-irradiated and regenerating rat thymus
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DOI：
10.1667/rr3331
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发表时间：
2005-04-01
影响因子：
3.4
通讯作者：
Fukumoto, T
中科院分区：
文献类型：
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作者：
Adachi, Y;Tokuda, N;Fukumoto, T
根据我们先前的观察，我们研究了在X-照射（8戈伊）和恢复的大鼠胸腺中来源于胸腺细胞或胸腺上皮细胞的细胞因子mRNA的表达（Mizutani等人，Radiat. Res. 157，281-289，2002）。检测白细胞介素2（112）、114、肿瘤坏死因子α（TNF）、干扰素γ（Ifng）和转化生长因子β（Tgfb）mRNA表达水平的变化。照射后第5天至第14天，112和114的mRNA表达下降。此后，112 mRNA的表达水平恢复到正常对照水平;然而，114 mRNA的表达趋向于显著低水平。TNF mRNA表达在照射后第5天下降，然后逐渐增加，恢复到正常对照水平。Tgfb mRNA表达无明显变化。从第11天到第14天，Ifng mRNA表达瞬时增强。I110 mRNA的表达水平在照射后第3 ~ 7天显著升高。此外，胸腺上皮细胞来源的I17的mRNA表达在第3天表现出短暂的下降，然而，从第5天到第21天，它表现出持续增加，最终达到正常对照水平的两倍后，X射线照射。这些观察结果表明，细胞因子信息的表达在辐射胸腺显着改变，并没有恢复正常后很长一段时间后，8戈伊照射。(c)2005年，辐射研究协会。
We investigated the expression of cytokine mRNA derived from thymocytes or thymic epithelial cells in X-irradiated (8 Gy) and recovering rat thymuses, according to our previous observation (Mizutani et al, Radiat. Res. 157, 281-289, 2002). The changes in mRNA expression level of interleukin 2 (112), 114, tumor necrosis factor alpha (Tnf), interferon gamma (Ifng), and transforming growth factor beta (Tgfb) were examined. The mRNA expression of 112 and 114 decreased from day 5 to day 14 after irradiation. Thereafter, the expression level of 112 mRNA recovered to normal control levels; however, the expression of 114 mRNA tended toward significantly low levels. Tnf mRNA expression decreased on day 5 after irradiation and then showed a gradual increase back to normal control levels. Tgfb mRNA expression did not change significantly. Ifng mRNA expression was transiently enhanced from day 11 to day 14. The mRNA expression levels of I110 increased significantly from day 3 to day 7 after irradiation. In addition, the mRNA expression of thymic epithelial cell-derived I17 showed a transient decrease on day 3; however, then it showed a continuous increase from day 5 to day 21, finally reaching twice the normal control levels after X irradiation. These observations suggest that the expression of cytokine messages in the irradiated thymus changed significantly and did not return to normal for a long time after 8 Gy irradiation. (c) 2005 by Radiation Research Society.