A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model
A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model
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抑郁症合并肝郁脾虚证大鼠模型
DOI：
10.1002/ar.24388
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发表时间：
2020-04-30
影响因子：
2
通讯作者：
Chen, Jia-Xu
中科院分区：
文献类型：
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作者：
Li, Xiao-Juan;Qiu, Wen-Qi;Chen, Jia-Xu
证在中医学疾病识别、诊断和治疗中起着至关重要的作用。肝郁脾虚证是临床上最常见的证型之一。在过去的几十年里，已经开发了几种动物模型来了解LQSSDS的潜在机制，但直到现在，该综合征的模拟仍然不清楚。近年来的研究证实，病证结合的动物模型是模拟中医证候的理想模型。重叠以前的研究报告，抑郁症是高度相关的LQSSDS，因此，我们试图建立一个大鼠模型相结合的抑郁症和LQSSDS。我们将大鼠暴露于不同持续时间的慢性不可预测的轻度应激（CIMIS）。随后，宏观评价指标包括旷场试验、蔗糖偏好试验、强迫游泳试验等行为学试验、摄食量、体重、白色脂肪组织、粪便含水量、内脏高敏感性、小肠转运等;微观评价指标包括下丘脑-垂体-肾上腺轴的变化。使用血清D-木糖吸收来全面确认和评估该模型在CUMS诱导过程中是否成功。结果表明，暴露于6周的BOS程序的大鼠表现出与LQSSDS和抑郁症表型显著相似的性状。这项研究为LQSSDS提供了一种新的大鼠模型，并可能导致更好地了解LQSSDS的病理生理学和开发这种综合征的新药。
A syndrome (Zheng in Chinese) plays a critical role in disease identification, diagnosis, and treatment in traditional Chinese medicine (TCM). Clinically, the liver Qi stagnation and spleen deficiency syndrome (LQSSDS) is one of the most common syndrome patterns. Over the past few decades, several animal models have been developed to understand the potential mechanisms of LQSSDS, but until now, simulation of the syndrome is still unclear. Recently, several studies have confirmed that an animal model combining a disease and a syndrome is appropriate for simulating TCM syndromes. Overlapping previous studies have reported that depression is highly associated with LQSSDS; hence, we attempted to develop a rat model combining depression and LQSSDS. We exposed the rats to different durations of chronic unpredictable mild stress (CUMS). Subsequently, the evaluation indicators at macrolevel consisted of behavioral tests including open field test, sucrose preference test, and forced swim test, food intake, body weight, white adipose tissue, fecal water content, visceral hypersensitivity, and small bowel transit, and the evaluation indicators at microlevel included changes of hypothalamic–pituitary–adrenal axis. Serum D‐xylose absorption was used to comprehensively confirm and assess whether the model was successful during the CUMS‐induced process. The results showed that rats exposed to 6‐week CUMS procedure exhibited significantly similar traits to the phenotypes of LQSSDS and depression. This study provided a new rat model for the LQSSDS and could potentially lead to a better understanding of the pathophysiology of LQSSDS and the development of new drugs for this syndrome.