Genome sequence of the plant pathogen Ralstonia solanacearum
Genome sequence of the plant pathogen Ralstonia solanacearum
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DOI：
10.1038/415497a
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发表时间：
2002-01-31
期刊：
影响因子：
64.8
通讯作者：
Boucher, CA
中科院分区：
文献类型：
--
作者：
Salanoubat, M;Genin, S;Boucher, CA
青枯雷尔氏菌是一种毁灭性的土传植物病原菌，具有全球性分布和异常广泛的宿主范围。这是一个模型系统的解剖分子决定因素的致病性。本文报道了GMI1000菌株的全基因组序列及其分析结果。5.8兆碱基（Mb）的基因组被组织成两个复制子：3.7兆碱基的染色体和2.1兆碱基的大质粒。这两种复制子都具有嵌合结构，为通过水平基因转移获得基因提供了证据。含有与G+C偏性百分比相关的遗传移动的元件的区域可能在基因组进化中具有重要功能。基因组编码许多蛋白质，可能与致病作用有关。特别是，许多假定的附件因素被确定。可以研究III型分泌的效应蛋白的完整库。确定了40多名候选人。与其他基因组的比较表明，细菌性植物病原体和动物病原体的港口不同阵列的专门III型依赖效应。
Ralstonia solanacearum is a devastating, soil-borne plant pathogen with a global distribution and an unusually wide host range. It is a model system for the dissection of molecular determinants governing pathogenicity. We present here the complete genome sequence and its analysis of strain GMI1000. The 5.8-megabase (Mb) genome is organized into two replicons: a 3.7-Mb chromosome and a 2.1-Mb megaplasmid. Both replicons have a mosaic structure providing evidence for the acquisition of genes through horizontal gene transfer. Regions containing genetically mobile elements associated with the percentage of G+C bias may have an important function in genome evolution. The genome encodes many proteins potentially associated with a role in pathogenicity. In particular, many putative attachment factors were identified. The complete repertoire of type III secreted effector proteins can be studied. Over 40 candidates were identified. Comparison with other genomes suggests that bacterial plant pathogens and animal pathogens harbour distinct arrays of specialized type III-dependent effectors.