Analysis of proteinase A function in yeast.
Analysis of proteinase A function in yeast.
复制标题
酵母中蛋白酶A功能的分析。
DOI：
10.1111/j.1432-1033.1981.tb06427.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Dieter H. Wolf
中科院分区：
文献类型：
--
作者：
B. Mechler;Dieter H. Wolf
分离缺乏蛋白酶A的酵母突变体。这些突变体之一（血红蛋白I）的特点是详细。突变pra1在减数分裂四分体中以2：2分离，表明是单基因突变。未检出抗（蛋白酶A）交叉反应物质。pra1杂合子二倍体显示基因剂量。因此，PRA1可能是蛋白酶A的结构基因。用这种突变体获得的结果表明，蛋白酶A不是营养细胞周期的重要组成部分。突变体在丰富和贫穷的生长条件下表现出正常的有丝分裂生长，并显示正常交配。受碳分解代谢物失活和NADP依赖性谷氨酸脱氢酶失活影响的酶，被认为具有蛋白水解性质，不受蛋白酶A缺乏的影响。然而，孢子形成条件下的蛋白质降解在蛋白酶A突变体细胞中减少约30%。孢子形成的分化过程也受到干扰，导致突变体细胞中孢子形成频率降低40%。
Yeast mutants lacking proteinase A were isolated. One of these mutants (Hb I) is characterized in detail. The mutation called pra1 segregates 2:2 in meiotic tetrads indicating a single gene mutation. No anti-(proteinase A) cross-reacting material can be detected. Diploids heterozygous for pra1 show gene dosage. Thus, it appears that PRA1 might be the structural gene for proteinase A. Results obtained with this mutant show that proteinase A is not a vital component of the vegetative cell cycle. The mutant exhibits normal mitotic growth under rich and poor growth conditions and shows normal mating. Enzymes subject to carbon catabolite inactivation and inactivation of NADP-dependent glutamate dehydrogenase, processes which were proposed to be of proteolytic nature, are not affected by the absence of proteinase A. However, protein degradation under sporulation conditions is about 30% reduced in proteinase A mutant cells. The differentiation process of sporulation is also disturbed leading to a 40% reduced sporulation frequency in mutant cells.
酿酒酵母蛋白酶缺陷突变体的蛋白质降解、减数分裂和孢子形成。
影响因子：
3.3
作者：
Zubenko,GS;Jones,EW
通讯作者：
Jones,EW
酿酒酵母蛋白酶缺陷突变体中 NADP 依赖性谷氨酸脱氢酶的蛋白水解失活。
影响因子：
3.9
作者：
Hemmings,BA;Zubenko,GS;Jones,EW
通讯作者：
Jones,EW