arfA antisense RNA regulates MscL excretory activity
arfA antisense RNA regulates MscL excretory activity
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DOI：
10.1101/2022.11.21.517365
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发表时间：
2022-11
影响因子：
4.4
通讯作者：
R. Morra;Fenryco Pratama;Thomas M. Butterfield;Geizecler Tomazetto;Kate Young;R. López;N. Dixon
中科院分区：
文献类型：
--
作者：
R. Morra;Fenryco Pratama;Thomas M. Butterfield;Geizecler Tomazetto;Kate Young;R. López;N. Dixon
细菌通过使用参与各自应激反应途径的基因之间的反义RNA串扰来协调它们如何响应翻译和渗透应激。细胞质蛋白（ECP）的排泄是一种常见的现象，在细菌中，这种部分细胞外定位的细胞内蛋白质组已牵连在各种应激反应机制。在响应低渗休克和核糖体失速在大肠杆菌，ECP是依赖于存在的大电导机械敏感通道和替代核糖体拯救因子A基因产物。然而，它是不知道的，如果一个机械的联系之间存在相应的基因和各自的应激反应途径。在这里，我们报告说，相应的mscL和arfA基因通常共同位于γ-变形菌的基因组和显示重叠在各自的3′ UTR和3′ CDS。我们发现这种不寻常的基因组排列允许mscL和arfA之间的反义RNA介导的调节控制，这调节了E.杆菌这些发现强调了E. coli中，进一步阐明了arfA sRNA的未知调控功能。
Bacteria coordinate how they respond to translation and osmotic stress by using antisense RNA crosstalk between the genes involved in the respective stress response pathways. Excretion of cytoplasmic protein (ECP) is a commonly observed phenomenon in bacteria, and this partial extracellular localisation of the intracellular proteome has been implicated in a variety of stress response mechanisms. In response to hypoosmotic shock and ribosome stalling in Escherichia coli, ECP is dependent upon the presence of the large-conductance mechanosensitive channel and the alternative ribosome–rescue factor A gene products. However, it is not known if a mechanistic link exists between the corresponding genes and the respective stress response pathways. Here, we report that the corresponding mscL and arfA genes are commonly co-located on the genomes of Gammaproteobacteria and display overlap in their respective 3′ UTR and 3′ CDS. We show this unusual genomic arrangement permits an antisense RNA–mediated regulatory control between mscL and arfA, and this modulates MscL excretory activity in E. coli. These findings highlight a mechanistic link between osmotic, translational stress responses and ECP in E. coli, further elucidating the previously unknown regulatory function of arfA sRNA.