Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA.
Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA.
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单链腺病毒相关病毒基因组的证据：病毒 DNA 释放时形成 DNA 密度杂合体。
DOI：
10.1073/pnas.64.3.863
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发表时间：
1969
影响因子：
11.1
通讯作者：
F. Koczot
中科院分区：
文献类型：
--
作者：
J. Rose;K. Berns;M. Hoggan;F. Koczot
已知提取的腺病毒相关病毒DNA是双链的，因此，人们假设这些病毒颗粒包含双链基因组。然而，最近的发现表明，病毒颗粒中的DNA只相当于提取分子的一半。从含有溴脱氧尿苷取代的或未取代的DNA的病毒颗粒中提取的DNA的密度分析表明，病毒粒子含有单链DNA，当释放时，形成双链结构。类似的情况在其他病毒中还是未知的。
Extracted adenovirus-associated virus DNA is known to be double-stranded, and, therefore, it has been assumed that these virus particles contain a double-stranded genome. Recent findings, however, have suggested that the DNA in virus particles is equivalent to only half the molecular weight of extracted molecules. A density analysis of DNA extracted from a mixture of virus particles containing either bromodeoxyuridine-substituted or unsubstituted DNA shows that virions contain single-stranded DNA which, when released, forms duplex structures. A similar circumstance is as yet unknown among other viruses.