The role of the Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma
The role of the Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma
复制标题
DOI：
10.1056/nejmoa030847
复制
发表时间：
2003-12-25
影响因子：
158.5
通讯作者：
Shaughnessy, JD
中科院分区：
文献类型：
--
作者：
Tian, E;Zhan, FH;Shaughnessy, JD
背景：骨髓瘤细胞可能会分泌影响成骨细胞和/或破骨细胞功能的因子。方法：我们从新诊断的多发性骨髓瘤患者和对照组的骨髓中提取纯化的浆细胞，进行寡核苷酸芯片分析和生化和免疫组织化学分析，以确定溶骨损害的分子决定因素。结果：我们研究了45例对照组，36例磁共振成像(MRI)无法发现骨局灶性病变的多发性骨髓瘤患者和137例MRI检测到此类病变的多发性骨髓瘤患者。从纯化的10,000个骨髓瘤细胞中提取的大约10,000个基因中的57个的表达模式可以用来区分两组患者(P
Background: Myeloma cells may secrete factors that affect the function of osteoblasts, osteoclasts, or both.Methods: We subjected purified plasma cells from the bone marrow of patients with newly diagnosed multiple myeloma and control subjects to oligonucleotide microarray profiling and biochemical and immunohistochemical analyses to identify molecular determinants of osteolytic lesions.Results: We studied 45 control subjects, 36 patients with multiple myeloma in whom focal lesions of bone could not be detected by magnetic resonance imaging (MRI), and 137 patients in whom MRI detected such lesions. Different patterns of expression of 57 of approximately 10,000 genes from purified myeloma cells could be used to distinguish the two groups of patients (P