Latent human polyomavirus infection in pregnancy: investigation of possible transplacental transmission
Latent human polyomavirus infection in pregnancy: investigation of possible transplacental transmission
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DOI：
10.1080/00313020701716458
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发表时间：
2008-01-01
期刊：
影响因子：
4.5
通讯作者：
Monga, Guido
中科院分区：
文献类型：
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作者：
Boldorini, Renzo;Veggiani, Claudia;Monga, Guido
目的：研究人多瘤病毒JCV和BKV的经胎盘传播。方法：对300例孕妇的尿液和血液样本进行细胞学分析，寻找“诱饵细胞”，巢式PCR鉴定分离的多瘤病毒的存在和基因型，并对转录控制区进行序列分析。巢式PCR还用于研究所有新生儿的脐带血。结果：在1份尿样中检出诱骗细胞（1/300;0.33%）；80份尿样中检测到多瘤病毒DNA(26.6%)，其中28份尿样中检测到单独的BKV(9.3%)， 49份尿样中检测到单独的JCV(16.3%)， 3份尿样中同时检测到JCV-BKV（1%）。血液样本阳性17例（5.6%），对应于单独BKV 10例（3.3%），单独JCV 7例（2.3%）。仅在1株尿株中发现了转录控制区重排，包括在D区插入13bp，而在11株BKV和11株尿株中发现了点突变。序列分析显示，人20号染色体（5例）和14号染色体（3例）存在20bp的P片段（P42-61）插入；2株JCV为单bp点突变。在脐带血样本中寻找多瘤病毒DNA总是阴性的。结论：妊娠期经常检测到多瘤病毒DNA，而基因组重排罕见，未发现多瘤病毒经胎盘传播的证据。
Aims: The purpose of the study was to investigate the transplacental transmission of the human polyomaviruses JCV and BKV.Methods: Urine and blood samples from 300 pregnant women underwent cytological analysis to search for 'decoy cells', nested PCR to identify presence and genotype of isolated polyomaviruses, and sequence analysis of the transcription control region. Nested PCR was also used to study the umbilical cord blood of all their newborns.Results: Decoy cells were identified in only one urine sample (1/300; 0.33%); polyomavirus DNA was detected in 80 urine samples (26.6%) corresponding to BKV alone in 28 samples (9.3%), JCV alone in 49 samples (16.3%) and both JCV-BKV in three samples (1%). Blood samples were positive in 17 cases (5.6%), corresponding to BKV alone in 10 (3.3%), and JCV alone in 7 (2.3%). Rearrangements of the transcription control region were found in only one urinary JCV strain, consisting of the insertion of 13bp at D block, whereas point mutations were identified in 11 BKV and 11 JCV strains detected from urine. Sequence analysis of the BKV strains detected in blood samples revealed a 20bp insertion of P block (P42-61) in human chromosomes 20 (five cases) and 14 (three cases); two JCV strains had single bp point mutations. The search for polyomavirus DNA in umbilical cord blood samples was always negative.Conclusions: Polyomavirus DNA was frequently detected in pregnancy, whereas genomic rearrangements were rare, and no evidence of transplacental transmission of polyomavirus was obtained.