A critical role for interleukin-1 receptor accessory protein in interleukin-1 signaling
A critical role for interleukin-1 receptor accessory protein in interleukin-1 signaling
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DOI：
10.1002/eji.1830270139
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发表时间：
1997-01-01
影响因子：
5.4
通讯作者：
Falk, W
中科院分区：
文献类型：
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作者：
Korherr, C;Hofmeister, R;Falk, W
白细胞介素1(IL-1)是炎症和免疫反应的中心分子，其多效性是由I型IL-1受体(IL-1RI)介导的。与配体结合后，细胞表面的IL-1RI完全消失。我们发现，最近发现的IL-1RI辅助蛋白(IL-1RAcP)可以将沉默的细胞转化为功能齐全的IL-1RI复合体。尽管将IL-1RAcP转染到IL-1RAcP缺陷的EL4D6/76细胞中并没有改变I-125标记的IL-1α/IL-1RI复合体的结合动力学或解离常数，但成功地恢复了激活的受体复合体的内化和IL-1刺激的IL-2产生的非常早期的事件。因此，IL-1RAcP的募集是IL-1RI激活复合体介导的信号级联反应的关键早期步骤。
Interleukin-1 (IL-1) is a central molecule in inflammation and immune responses whose pleiotropic activities are mediated by the type I IL-1 receptor (IL-1RI). The IL-1RI alone on the cell surface is silent after binding of the ligand. We show that the recently identified IL-1RI accessory protein (IL-1RAcP) converts the silent into a fully functional IL-1RI complex. Although transfection of IL-1RAcP into IL-1RAcP-deficient EL4D6/76 cells did not alter the binding kinetics or dissociation constants of the I-125-labeled IL-1 alpha/IL-1RI complex, a very early event, internalization of the activated receptor complex, and a late event, IL-1-stimulated IL-2 production, were successfully restored. Therefore, recruitment of IL-1RAcP is a critical early step in the signaling cascade mediated by the IL-1RI activation complex.