In vivo quantitative determination of the concentration of the polymerization agent methyl 2-benzoylbenzoate in intravenous injection solution and the cytotoxic effects of the chemical on normal human peripheral blood mononuclear cells.
In vivo quantitative determination of the concentration of the polymerization agent methyl 2-benzoylbenzoate in intravenous injection solution and the cytotoxic effects of the chemical on normal human peripheral blood mononuclear cells.
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体内定量测定静脉注射液中聚合剂2-苯甲酰苯甲酸甲酯的浓度及其对正常人外周血单核细胞的细胞毒作用。
DOI：
10.1007/s11356-016-6332-y
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
T.
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文献类型：
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作者：
Tsuboi;C.;Kawasaki;Y.;Yoshitome;K.;Yagi;K.;Miura;T.;Esumi;S.;Miyazaki;I.;Asanuma;M.;Kiyamura;Y. and Sendo;T.
在以前的研究中，我们检测到光引发剂1-羟基环己基苯基酮（1-HCHPK）和2-甲基-4 '-（甲硫基）-2-吗啉基苯丙酮（MTMP）在静脉注射液。重要的是，1-HCHPK和MTMP已被证明对正常人外周血（PB）单核细胞（MNC）具有细胞毒性。细胞死亡（凋亡）途径可分为两种模式，半胱天冬酶依赖性途径和非依赖性途径。然而，目前尚不清楚2-苯甲酰苯甲酸甲酯（MBB）是否诱导正常人PBMNC中的半胱天冬酶依赖性和/或非依赖性途径。在目前的体外研究中，我们研究了MBB的水平在一个解决方案，从静脉输液袋和MBB对正常人PBMNC的细胞毒性，通过半胱天冬酶-8-，半胱天冬酶-9-，或凋亡诱导因子（AIF）介导的细胞凋亡途径。我们发现进样溶液的浸提液被约80 μM的光引发剂MBB污染。此外，MBB在体外高浓度范围内诱导正常人PBMNC凋亡。此外，我们发现MBB诱导的细胞凋亡通过caspase-9途径发生，而不是AIF途径。总之，我们认为MBB在体外对正常人PBMNC具有细胞毒性作用，这是通过caspase依赖性途径介导的。
In previous studies, we detected the photoinitiators 1-hydroxycyclohexyl phenyl ketone (1-HCHPK) and 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) in an intravenous injection solution. Importantly, 1-HCHPK and MTMP have been demonstrated to be cytotoxic to normal human peripheral blood (PB) mononuclear cells (MNC). Cell death (apoptosis) pathways can be classified into two modes, caspase-dependent and -independent pathways. However, it is unclear whether methyl 2-benzoylbenzoate (MBB) induces the caspase-dependent and/or -independent pathway in normal human PBMNC. In the present in vitro study, we examined the levels of MBB in a solution from an intravenous fluid bag and the cytotoxicity of MBB towards normal human PBMNC via the caspase-8-, caspase-9-, or apoptosis-inducing factor (AIF)-mediated apoptosis pathways. We found that extracts from the injection solution had been contaminated with approximately 80 μM of the photoinitiator MBB. In addition, MBB induced apoptosis in the high concentration range in normal human PBMNC in vitro. Moreover, we found that MBB-induced apoptosis occurs via the caspase-9 pathway, but not the AIF pathway. In conclusion, we suggest that MBB has cytotoxic effects on normal human PBMNC in vitro, which are mediated via the caspase-dependent pathway.