Molecular evidence of new variant Brucella in North Pacific common minke whales
Molecular evidence of new variant Brucella in North Pacific common minke whales
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DOI：
10.1016/j.micinf.2004.06.006
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发表时间：
2004-11-01
影响因子：
5.8
通讯作者：
Maruyama, T
中科院分区：
文献类型：
--
作者：
Ohishi, K;Takishita, K;Maruyama, T
布鲁氏菌是近年来从多种海洋哺乳动物中分离到的一种布鲁氏菌病病原体。海洋哺乳动物布鲁氏菌菌株的分子分析，没有明显的病理布鲁氏菌病在北大西洋东部，表明他们是从陆地布鲁氏菌物种不同。以前，我们报告了异常的性腺在西北太平洋的小须鲸（Balaenoptera acutorostrata），并建议存在布鲁氏菌感染的鲸鱼在病理学和血清学研究。在本研究中，使用聚合酶链反应（PCR），布鲁氏菌检测显示干酪化或钙化的鲸鱼颗粒睾丸。结果表明，从北太平洋小须鲸分离的布鲁氏菌与陆地和北大西洋海洋哺乳动物分离的布鲁氏菌不同。在已知的布鲁氏菌菌株中，北太平洋布鲁氏菌与北大西洋海豹菌株的相似性最高。(C)2004年，Elsevier SAS。All rights reserved.
Brucella, a causative agent of brucellosis, has been isolated recently from a variety of marine mammals. The molecular analysis of marine mammalian Brucella strains, without manifest pathology of brucellosis in the eastern North Atlantic, showed that they are distinct from terrestrial Brucella species. Previously, we reported abnormal gonads in common minke whales (Balaenoptera acutorostrata) in the western North Pacific and suggested the presence of Brucella infection in the whales in pathology and serology studies. In the present study, using polymerase chain reaction (PCR), Brucella was detected in granular testes of the whales showing caseation or calcification. The insertion of an IS711 transposable element specific for marine mammal isolates as well as a seal isolate-specific DNA fragment were also found. Molecular characterization of Brucella based on sequence analysis of the PCR products amplified from the outer membrane protein (omp) 2 gene showed that the Brucella from North Pacific common minke whales was different from terrestrial and North Atlantic marine mammal Brucella strains. The North Pacific Brucella showed the highest similarity to North Atlantic seal strains among the known Brucella strains. (C) 2004 Elsevier SAS. All rights reserved.