Cutting Edge: Distinct Waves of BCL6 Expression during T Follicular Helper Cell Development
Cutting Edge: Distinct Waves of BCL6 Expression during T Follicular Helper Cell Development
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DOI：
10.4049/jimmunol.1101393
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发表时间：
2011-09-01
影响因子：
4.4
通讯作者：
Ansel, K. Mark
中科院分区：
文献类型：
--
作者：
Baumjohann, Dirk;Okada, Takaharu;Ansel, K. Mark
滤泡辅助性T细胞（T（FH））是T细胞依赖性体液免疫应答的发展和调节的中心。转录抑制因子BCL 6是T（FH）反应所必需的，但BCL 6蛋白在活化的CD 4（+）T细胞中表达的动力学尚未建立。我们使用细胞内染色和YFP-BCL 6融合蛋白报告小鼠在单细胞水平上测量了T（FH）细胞发育过程中BCL 6的表达。在树突状细胞-T细胞相互作用期间，BCL 6在所有分裂的T细胞中立即上调。第二波早期BCL 6表达与CXCR 5的诱导相一致，导致了独特且稳定的T（FH）细胞群。同源B细胞不是诱导BCL 6所必需的，但支持T（FH）细胞的扩增。这些数据表明，BCL 6参与了T（FH）细胞发育的非常早期的事件，并且与APC的反复接触增强了BCL 6的表达，从而建立了T（FH）细胞表型。免疫学杂志，2011，187：2089-2092。
T follicular helper (T(FH)) cells are central to the development and regulation of T cell-dependent humoral immune responses. The transcriptional repressor BCL6 is required for T(FH) responses, but the kinetics of BCL6 protein expression in activated CD4(+) T cells have not been established. We measured BCL6 expression during T(FH) cell development at the single-cell level using intracellular staining and YFP-BCL6 fusion protein reporter mice. BCL6 was immediately upregulated in all dividing T cells during dendritic cell-T cell interactions. A second wave of early BCL6 expression coincided with the induction of CXCR5, resulting in a distinct and stable T(FH) cell population. Cognate B cells were not required for the induction of BCL6, but supported the expansion of T(FH) cells. These data suggest that BCL6 participates in very early events in T(FH) cell development, and that repeated encounters with APCs reinforce BCL6 expression, thereby establishing the T(FH) cell phenotype. The Journal of Immunology, 2011, 187: 2089-2092.