DISTRIBUTION OF TYPE-X COLLAGEN MESSENGER-RNA IN NORMAL AND OSTEOARTHRITIC HUMAN CARTILAGE
DISTRIBUTION OF TYPE-X COLLAGEN MESSENGER-RNA IN NORMAL AND OSTEOARTHRITIC HUMAN CARTILAGE
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DOI：
10.1016/0169-6009(91)90005-k
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发表时间：
1991-11-01
期刊：
影响因子：
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通讯作者：
FREEMONT, AJ
中科院分区：
文献类型：
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作者：
HOYLAND, JA;THOMAS, JT;FREEMONT, AJ
胶原X是一种由肥大软骨细胞合成的独特同源三聚体，其功能尚不清楚，但其在钙化部位的定位和瞬时表达表明其可能与软骨内骨形成早期阶段的事件有关。 骨关节炎（OA）是一种以新骨形成为特征的疾病，但X型胶原在其发病机制中的作用尚不清楚。 一个700 bp的限制性片段编码的大部分的C-末端非胶原结构域和部分的3 '-非翻译区的人胶原X基因已被用于原位杂交研究的人OA关节从髋关节和膝关节置换手术，并与X型胶原基因产物的免疫组织化学定位的结果进行比较。 在OA组织中的软骨细胞中检测到X型胶原基因表达，这些软骨细胞似乎重新启动了软骨内骨形成过程，包括骨赘和软骨下骨硬化区域。
The function of collagen X, a unique homotrimer synthesised by hypertrophic chondrocytes, is not known but its localisation and transient expression at sites of calcification suggest that it is likely to be associated with events in the early stages of endochondral bone formation. Osteoarthritis (OA) is a disorder characterised by new bone formation but the role of type X collagen in its pathogenesis is unclear. A 700-bp restriction fragment encoding most of the C-terminal non-collagenous domain and part of the 3'-untranslated region of the human collagen X gene has been used for in situ hybridisation studies on human OA joints removed from hip and knee replacement operations and the results compared with immunohistochemical localisation of type X collagen gene product. Collagen X gene expression was detected in chrondrocytes present in OA tissue in areas where there appeared to be a re-initiation of the endochondral bone formation process including osteophytes and areas of subchondral bone sclerosis.