A new antimicrobial antibiotic from Actinoplanes capillaceus sp. K95-5561T.
A new antimicrobial antibiotic from Actinoplanes capillaceus sp. K95-5561T.
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DOI：
10.7164/antibiotics.53.1212
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发表时间：
2000-10
期刊：
影响因子：
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通讯作者：
A. Fukami;T. Nakamura;K. Kawaguchi;M. Rho;A. Matsumoto;Y. Takahashi;K. Shiomi;M. Hayashi;
中科院分区：
文献类型：
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作者：
A. Fukami;T. Nakamura;K. Kawaguchi;M. Rho;A. Matsumoto;Y. Takahashi;K. Shiomi;M. Hayashi;
deposited at Japan Collection of Microorganisms and the Institute for Fermentation, Osaka under accession number 10268T and 16408T, respectively. Detailed taxonomic study of the strain K95-55611 will be reported elsewhere3). A stock culture of the producing organism was inoculated into two 500-ml Erlenmeyer flasks containing 100ml of culturing medium. The flasks were incubated at 27°C for 72 hours on a rotary shaker (210rpm). Then, 5 ml portions of the growth were transferred into thirty 500-ml Erlenmeyer flasks containing 100 ml of the same medium. The flasks were incubated at 27°C for 72 hours on a rotary shaker, and 3 liters of the resulting culture was transferred into a 90-liter jar fermentor containing 60 liters of the same mediumas described above. The fermentation was carried out at 27°C for 6 days using an agitation rate of 210 rpm and an aeration rate of 60 liters per minute. The fermentation broth ofActinoplanes capillaceus K955561T (60 liters) was extracted with the same volume of EtOAc, and the EtOAc layer was dried up in vacuo to yield a brown oil (7.4g). This brown oil was subjected to a silica
