Complications of IgA nephropathy in a non-insulin-dependent diabetes model, the Akita mouse
Complications of IgA nephropathy in a non-insulin-dependent diabetes model, the Akita mouse
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DOI：
10.1620/tjem.198.233
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发表时间：
2002-12-01
影响因子：
2.2
通讯作者：
Koizumi, A
中科院分区：
文献类型：
--
作者：
Haseyama, T;Fujita, T;Koizumi, A
在胰岛素2基因（Ins2（秋田））中有突变（Cys96TyT）的秋田小鼠是糖尿病的模型。雄性秋田鼠患有糖尿病，而雌性患有轻度糖尿病。本研究旨在研究秋田小鼠模型的肾脏并发症，该模型一直保持在C57BL/6背景（B6）的Ins2(Akita)（+/-）杂合状态。研究了B6背景下糖尿病小鼠和对照组小鼠的肾功能（BUN、肌酐）、血清IgA浓度和肾脏组织学变化。在10、20、30和40周龄时，每组雄性和雌性小鼠各5只（糖尿病与对照组）。采用C3H/He （C3H）背景下的雄性糖尿病小鼠，检测了主要组织相容性抗原（MHC）对肾脏并发症的影响。与对照组相比，30周龄后，患有糖尿病的男性（而非女性）出现了肾功能受损，血清IgA升高。糖尿病性肾小球硬化随着年龄的增长而发展。20周后用免疫荧光显微镜检测糖尿病男性系膜弥漫性颗粒状IgA沉积。我们使用C3H背景的男性糖尿病患者测试了iga相关病变是否受MHC的影响。在C3H和B6背景小鼠中发现了相似程度的糖尿病肾小球硬化和肾小球IgA沉积。秋田小鼠是一种独特的小鼠模型，同时显示系膜硬化和IgA肾病。- C57BL/6秋田鼠；糖尿病性肾小球硬化症;肾小球IgA沉积；MHC (C) 2002东北大学医学出版社。
The Akita mouse, which has a mutation (Cys96TyT) in the insulin 2 gene (Ins2(Akita)), is a model for diabetes. The male Akita mouse has diabetes, while females develop mild diabetes. This study aimed to investigate renal complications in the Akita mouse model, which has been maintained in a heterozygous state Ins2(Akita) (+/-) with a C57BL/6 background (B6). Renal function (BUN, creatinine), serum IgA concentrations and histological changes in the kidneys were evaluated in diabetic and control mice in a B6 background. Five each of male and female mice per group (diabetes vs. control) were killed at 10, 20, 30 and 40 weeks of age. The influence of major histocompatibility antigens (MHC) on renal complications was tested using male diabetic mice in a C3H/He (C3H) background. When compared with controls, diabetic males, but not females, developed impaired renal function with elevation of serum IgA after 30 weeks of age. Diabetic glomerulosclerosis advanced with age in both sexes. Diffuse granular mesangial deposits of IgA were detected by immunofluorescence microscopy in diabetic males after 20 weeks. We tested whether the IgA-associated lesions were influenced by MHC using diabetic males in a C3H background. Similar degrees of diabetic glomerulosclerosis and glomerular IgA deposition were found in mice with C3H and B6 backgrounds. Akita mouse is a unique mouse model showing both mesangial sclerosis and IgA nephropathy. - C57BL/6 Akita mouse; diabetic glomerulosclerosis; glomerular IgA deposition; MHC (C) 2002 Tohoku University Medical Press.