Peroxisomal Membrane Protein Pmp47 Is Essential in the Metabolism of Middle-chain Fatty Acid in Yeast Peroxisomes and Is Associated with Peroxisome Proliferation*
Peroxisomal Membrane Protein Pmp47 Is Essential in the Metabolism of Middle-chain Fatty Acid in Yeast Peroxisomes and Is Associated with Peroxisome Proliferation*
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过氧化物酶体膜蛋白 Pmp47 对于酵母过氧化物酶体中链脂肪酸的代谢至关重要，并且与过氧化物酶体增殖相关*
DOI：
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发表时间：
2000
影响因子：
4.8
通讯作者：
Y. Sakai
中科院分区：
文献类型：
--
作者：
T. Nakagawa;T. Imanaka;M. Morita;Kazuhiko Ishiguro;H. Yurimoto;A. Yamashita;N. Kato;Y. Sakai
甲基营养酵母 Candida boidinii 的 Pmp47 属于溶质转运蛋白的线粒体家族，位于过氧化物酶体膜中。其人类同源物 Pmp34 也是已知的。在这项研究中，我们使用 PMP47 缺失的 C. boidinii 菌株（菌株 pmp47Δ）描述了 Pmp47 在脂肪酸代谢和过氧化物酶体增殖中的作用。野生型菌株以中链脂肪酸月桂酸酯为单一碳源生长良好，在生长过程中观察到轻度的过氧化物酶体增殖。 pmp47Δ菌株不能在月桂酸上生长，但可以在长链脂肪酸（包括棕榈酸、肉豆蔻酸和油酸）上生长。菌株pmp47Δ的完整细胞和半透化细胞中的月桂酸氧化活性水平低于野生型菌株中的相应水平，尽管菌株pmp47Δ的细胞裂解物中的月桂酸氧化活性水平和半透化细胞中的月桂酰辅酶A氧化水平与野生型菌株中的相应水平没有区别。当通过表达其过氧化物酶体靶向信号被删除的酿酒酵母Faa2p(月桂酰辅酶A合成酶)在菌株pmp47Δ的细胞质中提供月桂酰辅酶A时，菌株pmp47Δ在月桂酸上的生长恢复至野生型菌株的生长水平。月桂酸酯在过氧化物酶体中通过月桂酰辅酶 A 合成酶的作用转化为其辅酶 A 形式。这些结果表明，Pmp47 参与过氧化物酶体中月桂酸转化为其 CoA 形式所需的小分子（可能是 ATP）的运输，并且 Pmp47 的缺失会导致月桂酸代谢受损，从而导致 pmp47Δ 细胞无法在月桂酸上生长。此外，Pmp47可能参与过氧化物酶体增殖，因为从表达带有过氧化物酶体靶向信号1（GFP-AKL）标记的绿色荧光蛋白的细胞的荧光分析判断，pmp47Δ菌株含有减少数量的过氧化物酶体。
Pmp47 of the methylotrophic yeast Candida boidinii belongs to a mitochondrial family of solute transporters and is localized in peroxisomal membranes. Its human homolog, Pmp34, is also known. In this study, we characterized the role of Pmp47 in fatty acid metabolism and peroxisome proliferation using thePMP47-deleted strain of C. boidinii (strainpmp47Δ). The wild-type strain grew well on a middle-chain fatty acid, laureate, as the single carbon source, and mild peroxisome proliferation was observed during its growth. The pmp47Δstrain could not grow on laureate but could grow on long-chain fatty acids including palmitate, myristate, and oleate. The levels of laureate oxidation activity in intact cells and in semi-permeabilized cells of strain pmp47Δ were lower than the respective level in the wild-type strain, although the level of laureate oxidation activity in the cell lysate and the level of lauroyl-CoA oxidation in semi-permeabilized cells of strain pmp47Δ were indistinguishable from the respective level in the wild-type strain. When lauroyl-CoA was provided in the cytosol of strainpmp47Δ through expression of Saccharomyces cerevisiae Faa2p (lauroyl-CoA synthetase) in which its peroxisome targeting signal was deleted, the growth of strain pmp47Δon laureate was recovered to the level of growth of the wild-type strain. Laureate is converted to its CoA form in peroxisomes by the action of lauroyl-CoA synthetase. These results suggested that Pmp47 is involved in the transport of a small molecule (possibly ATP) required in the conversion of laureate to its CoA form in peroxisomes and that the absence of Pmp47 causes impairment of laureate metabolism, which results in the inability of pmp47Δ cells to grow on laureate. In addition, Pmp47 may be involved in peroxisome proliferation, because the pmp47Δ strain contained a reduced number of peroxisomes, as judged from the fluorescence analysis of cells expressing green fluorescent protein tagged with the peroxisome targeting signal 1 (GFP-AKL).
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三种酿酒酵母酰基辅酶 A 合成酶 Faa1p、Faa2p 和 Faa3p 的生化研究。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Knoll,LJ;Johnson,DR;Gordon,JI
通讯作者：
Gordon,JI
DOI：
10.1006/bbrc.1999.0510
发表时间：
1999-04-13
影响因子：
3.1
作者：
Steinberg, SJ;Wang, SJ;Watkins, PA
通讯作者：
Watkins, PA
DOI：
10.1073/pnas.93.7.2646
发表时间：
1996-04-02
影响因子：
11.1
作者：
Ibrahimi, A;Sfeir, Z;Abumrad, NA
通讯作者：
Abumrad, NA
非洲爪蟾卵母细胞中非酯化脂肪酸可饱和转运系统的组成型表达。
DOI：
10.1042/bj2970315
发表时间：
1994
期刊：
The Biochemical journal
影响因子：
--
作者：
Zhou,SL;Stump,D;Isola,L;Berk,PD
通讯作者：
Berk,PD
DOI：
10.1016/s0021-9258(17)39756-9
发表时间：
1984-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J M Goodman;C W Scott;P N Donahue;J P Atherton
通讯作者：
J M Goodman;C W Scott;P N Donahue;J P Atherton