Rab35 is translocated from Arf6-positive perinuclear recycling endosomes to neurite tips during neurite outgrowth.
Rab35 is translocated from Arf6-positive perinuclear recycling endosomes to neurite tips during neurite outgrowth.
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DOI：
10.4161/sgtp.29290
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Fukuda, Mitsunori
中科院分区：
文献类型：
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作者：
Kobayashi, Hotaka;Etoh, Kan;Fukuda, Mitsunori
小GTP酶Rab35是神经突起生长的关键调节因子，它的激活显著增强了神经生长因子(NGF)诱导的神经突起生长。我们最近报道发现，Rab35及其效应分子在NGF刺激下，将囊泡形成的促进剂EHD1招募到Arf6阳性的核周循环内小体(以下简称循环内小体)。尽管Rab35很可能促进再生内小体运输囊泡的形成，从而促进轴突生长，但轴突生长过程中囊泡的去向尚不清楚。在这里，我们报告发现，在NGF刺激的晚期，Rab35从回收的内小体转移到轴突尖端。我们发现，在NGF刺激的前6小时，即NGF刺激的早期阶段，Rab35免疫荧光信号在循环内小体聚集，然后在NGF刺激的晚期(即，>；6 h至
Small GTPase Rab35 is a key regulator of neurite outgrowth, and its activation dramatically enhances nerve growth factor (NGF)-induced neurite outgrowth. We recently reported finding that Rab35 and its effector molecules recruit EHD1, a facilitator of vesicle formation, to Arf6-positive perinuclear recycling endosomes (hereafter simply referred to as recycling endosomes) in response to NGF stimulation. Although Rab35 is likely to promote the formation of transport vesicles from recycling endosomes that contributes to neurite outgrowth, the destination of the vesicles during neurite outgrowth remains unknown. Here we report finding that Rab35 is translocated from recycling endosomes to neurite tips in a late phase of NGF stimulation. We found that Rab35 immunofluorescence signals accumulated at recycling endosomes during the first 6 h, i.e., the early phase of NGF stimulation and then translocated to neurite tips during the late phase of NGF stimulation (i.e., >6 h to