Equivalent genetic roles for bmp7/snailhouse and bmp2b/swirl in dorsoventral pattern formation.
Equivalent genetic roles for bmp7/snailhouse and bmp2b/swirl in dorsoventral pattern formation.
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发表时间：
2000-03
期刊：
影响因子：
4.6
通讯作者：
Bettina Schmid;Maximilian Fürthauer;Stephanie A. Connors;Jamie Trout;B. Thisse;C. Thisse;M. Mullins
中科院分区：
文献类型：
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作者：
Bettina Schmid;Maximilian Fürthauer;Stephanie A. Connors;Jamie Trout;B. Thisse;C. Thisse;M. Mullins
骨形态发生蛋白（BMP）信号通路作用于脊椎动物胚胎的背腹轴的建立。在这里，我们证明了两种不同的BMP配体亚类基因的遗传需求，用于斑马鱼胚胎的背腹图形成。根据在BMP配体救援实验中观察到的相对效率，保守的染色体同步以及斑马鱼BMP7基因的分离，我们确定了强烈的背背突变体表型是由BMP7基因突变引起的。我们表明，原始的蜗牛等位基因是一个肌型突变，我们确定了蜗牛/bmp7 null突变体。我们证明，蜗牛/BMP7无效突变体表型与最强的背侧斑马鱼突变体漩涡/BMP2B的假定无效突变体表型相同，揭示了这两个BMP配体的等效遗传作用。双重突变蜗牛/BMP7;漩涡/BMP2B胚胎没有表现出额外或更强的背面表型，表明这些BMP配体在早期的胚胎发育中无法冗余。此外，过表达实验表明，BMP2B和BMP7通过细胞自主机制在野生型胚胎的腹侧化中协同作用，这表明BMP2B/BMP7异二聚体可以在体内作用于Zebrafish胚胎中的腹部细胞卵泡。
A bone morphogenetic protein (BMP) signaling pathway acts in the establishment of the dorsoventral axis of the vertebrate embryo. Here we demonstrate the genetic requirement for two different Bmp ligand subclass genes for dorsoventral pattern formation of the zebrafish embryo. From the relative efficiencies observed in Bmp ligand rescue experiments, conserved chromosomal synteny, and isolation of the zebrafish bmp7 gene, we determined that the strongly dorsalized snailhouse mutant phenotype is caused by a mutation in the bmp7 gene. We show that the original snailhouse allele is a hypomorphic mutation and we identify a snailhouse/bmp7 null mutant. We demonstrate that the snailhouse/bmp7 null mutant phenotype is identical to the presumptive null mutant phenotype of the strongest dorsalized zebrafish mutant swirl/bmp2b, revealing equivalent genetic roles for these two Bmp ligands. Double mutant snailhouse/bmp7; swirl/bmp2b embryos do not exhibit additional or stronger dorsalized phenotypes, indicating that these Bmp ligands do not function redundantly in early embryonic development. Furthermore, overexpression experiments reveal that Bmp2b and Bmp7 synergize in the ventralization of wild-type embryos through a cell-autonomous mechanism, suggesting that Bmp2b/Bmp7 heterodimers may act in vivo to specify ventral cell fates in the zebrafish embryo.