Expression of vitamin D receptor in lung cancer
Expression of vitamin D receptor in lung cancer
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DOI：
10.1007/bf01220803
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发表时间：
1996-06-01
影响因子：
3.6
通讯作者：
Havemann, K
中科院分区：
文献类型：
--
作者：
Kaiser, U;Schilli, M;Havemann, K
维生素D 1，25-二羟基胆钙化醇的活性代谢产物是一种调节细胞分化和增殖的类维生素D制剂。各种维生素D衍生物已显示诱导肿瘤细胞的分化。任何激素作用的先决条件是其受体的存在。我们研究了维生素D受体在人肺癌细胞系和原发性肺癌组织中的表达。采用聚合酶链反应，11个细胞系中有10个来自小细胞肺癌，15个来自非小细胞肺癌的细胞系中有15个表现出维生素D受体表达。用特异性单克隆抗体进行免疫组化分析，结果表明117例原发性小细胞肺癌中有31例（26%）有维生素D受体蛋白表达，阳性细胞呈核反应模式。在37例原发性非小细胞肺癌中，21例（特别是腺癌（9/14）和鳞状细胞癌（10/15））显示维生素D受体。结果表明，肺癌病例的一个子集可能容易受到维生素D类似物的分化作用。
The active metabolite of vitamin D 1,25-dihydroxycholecalciferol is a hormone-like agent that regulates cell differentiation and proliferation. Various vitamin D derivatives have been shown to induce differentiation in neoplastic cells. The prerequisite for any hormone action is the presence of its receptor. We studied the expression of vitamin D receptor in human lung cancer cell lines and in primary lung cancer tissue. Employing the polymerase chain reaction, 10 out of 11 cell lines stemming from small-cell lung cancer and 15 out of 15 cell lines stemming from non-small-cell lung cancer demonstrated vitamin D receptor expression. An immunohistochemical analysis, using a specific monoclonal antibody, demonstrated vitamin D receptor protein expression in 31 out of 117 (26%) primary small-cell lung cancer cases tested, Positive cells exhibited a nuclear reaction pattern. Twenty-one out of 37 primary non-small-cell lung cancer cases, particularly adenocarcinomas (9/14) and squamous-cell carcinomas (10/15), exhibited vitamin D receptor. Results indicate that a subset of lung cancer cases may be susceptible to the differentiating effects of vitamin D analogues.