Formation and capture of droplet with high volume ratio of cell to droplet
Formation and capture of droplet with high volume ratio of cell to droplet
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DOI：
10.1088/1361-6439/ac0a57
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发表时间：
2021
影响因子：
2.3
通讯作者：
Zhi Zhao;Zhen-Yu Xun;Liang-Liang Fan-Liang;J. Zhe;Liang Zhao
中科院分区：
文献类型：
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作者：
Zhi Zhao;Zhen-Yu Xun;Liang-Liang Fan-Liang;J. Zhe;Liang Zhao
细胞与液滴的高体积比对于通过将分子的浓度快速增加到可检测水平来快速检测分泌的分子是非常重要的。在本文中，小体积的卫星液滴，包裹细胞连续产生和捕获的T形微通道与分支通道，以实现高体积比的细胞液滴。通过实验系统地研究了连续相流量、通道几何形状和细胞浓度对卫星液滴形成的影响。结果表明，在细胞浓度为16.7%时，可产生最小的卫星液滴，其体积约为16.5 μm（约0.14 μ l），平均体积比可达45.9%（相对标准偏差为12.9%）。该方法具有连续操作和细胞与液滴体积比高的优点，可作为快速检测分泌型分子的先决条件。
High volume ratio of cell to droplet is of great importance for the fast detection on the secreted molecule by rapidly increasing the concentration of the molecule to the detectable level. In this paper, small-volume satellite droplet that encapsulates the cell is continuously generated and captured by a T-shaped microchannel with a branching channel to achieve high volume ratio of cell to droplet. The influence of the flow rate of the continuous phase, the channel geometry and the cell concentration on the formation of the satellite droplet is systematically studied by the experiment. The results show that the smallest satellite droplet with size of ∼6.5 μm (∼0.14 pl in volume) can be generated at a low cell concentration of 16.7% and an average volume ratio of cell to droplet can be achieved as high as ∼45.9% (the relative standard deviation is 12.9%). With advantages of continuous manipulation and high volume ratio of cell to droplet, the present method could be potentially utilized as a prerequisite step for the fast detection on the secreted molecule.