Transposon-tagged mutagenesis in the rat
Transposon-tagged mutagenesis in the rat
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DOI：
10.1038/nmeth1002
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发表时间：
2007-02-01
期刊：
影响因子：
48
通讯作者：
Takeda, Junji
中科院分区：
文献类型：
--
作者：
Kitada, Kazuhiro;Ishishita, Satoshi;Takeda, Junji
尽管实验室大鼠是生物医学研究和药物开发中不可或缺的实验动物，但缺乏胚胎干细胞系阻碍了基因敲除研究。在这里，我们报告了使用睡美人(SB)转座子系统成功地产生插入突变大鼠。这将使大鼠模型比小鼠模型更适合的各种生物医学研究领域受益。
Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.