Biogeneration of C13-norisoprenoid compounds:: experiments supportive for an apo-carotenoid pathway in grapevines
Biogeneration of C13-norisoprenoid compounds:: experiments supportive for an apo-carotenoid pathway in grapevines
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DOI：
10.1016/s0003-2670(01)01589-6
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发表时间：
2002-04-29
影响因子：
6.2
通讯作者：
Razungles, A
中科院分区：
文献类型：
--
作者：
Baumes, R;Wirth, J;Razungles, A
在葡萄中提出的从类胡萝卜素到C-13-norisoprenoids的生物发生途径的第一步涉及由区域特异性加氧酶对类胡萝卜素的酶促降解。化学，光化学和氧化酶偶联降解类胡萝卜素降异戊二烯类已在体外和酶系统研究表明，在哺乳动物中参与。然而，没有酶系统已被证明参与葡萄中C-13-降异戊二烯的形成，尽管最近对葡萄树进行的所有研究都支持这种模型。这些发现包括具有13个碳原子的类降异戊二烯的优势，从而表明裂解的特异性，C-13-类降异戊二烯和相应的类胡萝卜素共有的不对称中心和轴的构型，在浆果发育期间类降异戊二烯和类胡萝卜素水平之间观察到的负相关性，以及在转色和浆果成熟之间的浆果中C-13标记物从类胡萝卜素到去甲异戊二烯的体内转移。所有这些发现都是支持糖基化C-13-norisoprenoids来自葡萄浆果中的类胡萝卜素这一假设的主要论据。类胡萝卜素主要从果实形成的第一阶段开始合成，直到变熟，然后在变熟和成熟之间降解，产生糖基化的C-13-降异戊二烯和其他化合物。(C)2002 Elsevier Science B. V.保留所有权利。
The first step of the proposed biogenetic pathway in grapes that leads from carotenoids to C-13-norisoprenoids involves the enzymatic degradation of carotenoids by regiospecific oxygenases. Chemical, photochemical and oxidase-coupled degradation of carotenoids to norisoprenoids have been studied in vitro and enzymatic systems shown to be involved in mammals. However, no enzymatic system has been shown to be involved in the formation Of C-13-norisoprenoids in grapes, despite all recent studies carried out on grapevines supporting such a model. These findings include the preponderance of norisoprenoids possessing 13 carbon atoms that thereby indicates the specificity of cleavage, the configuration of the asymmetric centres and axes common to C-13-norisoprenoids and the corresponding carotenoids, the negative correlations observed between the levels of norisoprenoids and carotenoids during berry development, and the in vivo transfer of C-13 markers from carotenoids to norisoprenoids in berries between veraison and berry maturity. All of these findings are major arguments in favour of the hypothesis that glycosylated C-13-norisoprenoids derive from carotenoids in grape berries. Carotenoids are mostly synthesised from the first stage of fruit formation until veraison, and then degrade between veraison and maturity to produce glycosylated C-13-norisoprenoids and other compounds. (C) 2002 Elsevier Science B.V. All rights reserved.