Demonstration of rat CAR bacillus using a labelled streptavidin biotin (LSAB) method.
Demonstration of rat CAR bacillus using a labelled streptavidin biotin (LSAB) method.
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使用标记的链霉亲和素生物素 (LSAB) 方法演示大鼠 CAR 芽孢杆菌。
DOI：
10.1292/jvms.58.12_1219
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
A. Fernández
中科院分区：
文献类型：
--
作者：
J. Orós;S. Matsushita;J. L. Rodríguez;F. Rodríguez;A. Fernández
本文描述了采用基于标记链霉亲和素生物素（LSAB）方法和3-氨基-9-乙基咔唑（AEC）作为底物的免疫过氧化物酶技术，对石蜡包埋的实验感染大鼠肺中的大鼠CAR杆菌抗原进行免疫组织化学检测。免疫染色的模式仅限于纤毛支气管上皮细胞和这种技术的特异性得到证实。使用AEC作为底物被评价为比二氨基联苯胺（DAB）更有效。这种免疫过氧化物酶技术检测大鼠中的CAR芽孢杆菌的有用性和它的优点相比，间接免疫荧光（IF）进行了讨论。
Immunohistochemical detection of rat CAR bacillus antigen in paraffin-embedded experimentally infected rat lungs, using an immunoperoxidase technique based on the labelled streptavidin biotin (LSAB) method and 3-amino-9-ethylcarbazole (AEC) as substrate is described in this paper. The pattern of immunostaining was confined to the ciliated bronchial epithelium and the specificity of this technique was confirmed. The use of AEC as substrate was evaluated more efficient than diaminobenzidine (DAB). The usefulness of this immunoperoxidase technique for the detection of CAR bacillus in rats and its advantages compared to the indirect immunofluorescence (IF) are discussed.