Anatomical evidence of microbial biofilms in tonsillar tissues - A possible mechanism to explain chronicity
Anatomical evidence of microbial biofilms in tonsillar tissues - A possible mechanism to explain chronicity
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DOI：
10.1001/archotol.129.6.634
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发表时间：
2003-06-01
影响因子：
--
通讯作者：
Faddis, BT
中科院分区：
文献类型：
--
作者：
Chole, RA;Faddis, BT
背景：生物膜内的细菌对宿主防御和抗生素具有抗性。炎症扁桃体组织和隐窝内细菌生物膜的存在可以解释某些形式的扁桃体炎的慢性和复发特征。目的：确定临床异常扁桃体内是否存在微生物生物膜。设计：在这项研究中，我们评估了19个人类扁桃体的组织形态学外观，以利用光学和透射电子显微镜观察生物膜形成的证据。在手术扁桃体切除术期间收集人体组织。15例标本因反复感染而被移除，4例因肥大和梗阻而被移除。干预措施：在这项研究中没有使用干预措施主要结果测量：组织学和超微结构的证据，细菌生物膜内的隐窝扁桃体。结果：革兰氏阳性和革兰氏阴性细菌被视为在其他非细胞存款之间的隐窝11 15感染扁桃体。积累的细菌区域具有典型的无定形多糖生物膜基质的超微结构外观。细菌菌落的小集群被认为是在3 4扁桃体切除，因为hypertrophy.Conclusions：有很强的解剖学证据的细菌生物膜在慢性病扁桃体的存在。由于生物膜内的固着细菌对宿主防御和抗生素具有抗性，扁桃体内的细菌生物膜可以解释某些形式的扁桃体炎的慢性和复发性。
Context: Bacteria within biofilms are resistant to host defenses and antibiotics. The presence of bacterial biofilms within the tissue and crypts of inflamed tonsils may explain the chronicity and recurrent characteristics of some forms of tonsillitis.Objective: To determine if microbial biofilms occur within clinically abnormal tonsils.Design: In this study, we evaluated the histomorphological appearance of 19 human tonsils for evidence of biofilm formation using light and transmission electron microscopy.Subjects: Human tissues were collected during surgical tonsillectomy. Fifteen specimens were removed because of a history of repeated infections, and 4 were removed because of hypertrophy and obstruction. Interventions: No interventions were used in this studyMain Outcome Measure: Histological and ultrastructural evidence of bacterial biofilms within the crypts of tonsils.Results: Gram-positive and gram-negative bacteria were seen within otherwise acellular deposits among crypts of 11 of 15 infected tonsils. Regions of accumulated bacteria possessed the ultrastructural appearance of typical amorphous polysaccharide biofilm matrix. Small clusters of bacterial colonies were seen in 3 of 4 tonsils removed because of hypertrophy.Conclusions: There is strong anatomical evidence for the presence of bacterial biofilms in chronically diseased tonsils. Because sessile bacteria within biofilms are resistant to host defenses and antibiotics, bacterial biofilms within tonsils may explain the chronicity and recurrent nature of some forms of tonsillitis.