1-bit photon cross-correlation-based phase-modulation fluorometer using an OFDM signal-modulated excitation light source
1-bit photon cross-correlation-based phase-modulation fluorometer using an OFDM signal-modulated excitation light source
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DOI：
10.1088/1361-6501/ab0f77
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发表时间：
2019-06-01
影响因子：
2.4
通讯作者：
Miyauchi, Tomohiro
中科院分区：
文献类型：
--
作者：
Iwata, Tetsuo;Miyauchi, Tomohiro
我们提出了一种相位调制荧光计，这是一个1位光子互相关器构造使用现场可编程门阵列和激发光源调制的正交频分复用信号的组合。这种多路复用允许我们在给定的基带内有效地和选择性地使用多个调制频率。定义为其基频的倒数，复用的符号长度被设置为等于互相关器的时间窗口的宽度。因此，基带中的子载波的所有时间波形在符号间隔的两端平滑地连接;因此，在子载波的频域中不会出现杂散边带。1位互相关器在光子计数领域带来荧光寿命。根据荧光和参考信号之间的相位差光谱，可以同时精确地获得每个频率的荧光寿命。互相关器的分辨时间和时间窗口宽度分别为2.0 ns和256 ns。作为原理验证实验，我们测量了一些标准样品的荧光寿命。
We propose a phase-modulation fluorometer, which is a combination of a 1-bit photon cross-correlator constructed using a field-programmable gate array and an excitation light source modulated by an orthogonal frequency-division multiplexing signal. Such multiplexing allows us to use multiple modulation frequencies efficiently and selectively within a given baseband. Defined as the inverse of its fundamental frequency, the symbol length of the multiplexing is set equal to the width of the time window of the cross-correlator. Therefore, all temporal waveforms of the subcarriers in the baseband are connected smoothly at both ends of the symbol interval; hence, no spurious sidebands appear in the frequency domain of the subcarriers. The 1-bit cross-correlator brings about fluorescence lifetimes in the photon-counting realm. From a phase difference spectrum between the fluorescence and the reference signal, fluorescence lifetimes for each frequency may be obtained with precision simultaneously. The resolution time and the width of the time window of the cross-correlator are 2.0 ns and 256 ns, respectively. As a proof-of-principle experiment, we measured the fluorescence lifetimes of some standard samples.