Mechanical modulation of tenascin-C and collagen-XII expression during avian synovial joint formation
Mechanical modulation of tenascin-C and collagen-XII expression during avian synovial joint formation
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DOI：
10.1002/jor.1100180312
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发表时间：
2000-05-01
影响因子：
2.8
通讯作者：
Hunziker, EB
中科院分区：
文献类型：
--
作者：
Mikic, B;Wong, M;Hunziker, EB
本研究的目的是调查如何在没有正常功能的骨骼肌收缩在禽滑膜关节发育的特征性细胞外基质分子的时间和空间模式的改变。通过使用原位检测的蛋白质和mRNA的表达，在发展中的鸟类脚和股胫关节从广泛的发展阶段，我们表明，形态异常，导致胚胎制动与肌腱蛋白-C和胶原蛋白-XII的表达模式改变发展中的关节结构。当关节在固定胚胎中融合时，假定关节表面的细胞从扁平的成纤维细胞分化为更圆的软骨细胞，并且在完全关节融合的部位不再检测到胶原蛋白XII和I。虽然胶原蛋白XII的表达在固定的关节内的其他地方保持在正常水平，但腱生蛋白-C的表达在软骨骨骺、滑膜和肌腱内以及纤维关节面的剩余部分内减少。这种效应对于软骨骨骺内最短的腱蛋白变体（Tn 190）和肌腱、滑膜和关节纤维表面层内最大的变体（Tn 230）最为显著。因此，这项研究提供了在体内支持以前在体外的工作，表明腱生蛋白的表达是敏感的机械负荷环境的外部变化。然而，这些数据并不支持在早期发育过程中胶原蛋白XII的类似结论。
The objective of this study was to investigate how temporal and spatial patterns of characteristic extracellular matrix molecules are altered in the absence of normal functional skeletal muscle contractions during avian synovial joint development. By using in situ detection of protein and mRNA expression in developing avian feet and femorotibial joints from a wide range of developmental stages, we demonstrate that the morphological abnormalities that result from embryonic immobilization are associated with altered patterns of tenascin-C and collagen-XII expression within developing joint structures. As the joints fuse in immobilized embryos, the cells of the presumptive articular surface differentiate from flattened fibroblasts to more rounded chondrocytes and collagens XII and I are no longer detected at sites of complete joint fusion. Although the expression of collagen XII persists at normal levels elsewhere within the immobilized joint, tenascin-C expression is diminished within the chondroepiphysis, synovium, and tendons, as well as within the remains of the fibrous articular surface. This effect is most notable for the shortest tenascin variant (Tn190) within the chondroepiphysis and the largest variant (Tn230) within tendons, synovium, and the fibrous surface layer of the joint. This study thus provides in vivo support of previous in vitro work that suggests that tenascin expression is sensitive to external changes in mechanical loading environment. However, these data do not support a similar conclusion for collagen XII during early development.