Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary
Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary
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DOI：
10.1007/s13577-016-0142-x
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发表时间：
2016-08
期刊：
影响因子：
4.3
通讯作者：
A. Ohyama;Junko Toyomura;T. Tachibana;S. Isonishi;Haruka Takahashi;H. Ishikawa
中科院分区：
文献类型：
--
作者：
A. Ohyama;Junko Toyomura;T. Tachibana;S. Isonishi;Haruka Takahashi;H. Ishikawa
从患有卵巢透明细胞腺癌的患者的腹水建立了命名为NOCC的细胞系。该细胞系已连续生长，并通过连续传代（超过76次）连续繁殖超过7年。细胞呈球形至椭圆形，显示肿瘤性和多形性特征，以拼图样模式生长，同时形成单层，无接触抑制。细胞增殖迅速，但很容易漂浮成细胞片。种群倍增时间约为29 h。染色体数目从60到83不等。第30代染色体众数为70-74。NOCC细胞在3天的培养中分泌750.5ng/ml的VEGF。低氧诱导因子-1 α（HIF-1α）是低氧条件下VEGF的主要调节因子。NOCC细胞对抗癌药物BEV、DOX、GEM、ETP、CDDP或TXT不敏感。NOCC细胞移植到scid小鼠显示出与原始肿瘤相似的组织学方面。NOCC细胞及其移植物PAS反应均呈阳性。
A cell line, designated NOCC, was established from the ascites of a patient with clear cell adenocarcinoma of the ovary. The cell line has been grown without interruption and continuously propagated by serial passaging (more than 76 times) over 7 years. The cells are spherical to polygonal-shaped, display neoplastic, and pleomorphic features, and grow in a jigsaw puzzle-like pattern while forming monolayers without contact inhibition. The cells proliferate rapidly, but are easily floated as a cell sheet. The population doubling time is about 29 h. The number of chromosomes ranges from 60 to 83. The modal number of chromosomes is 70–74 at the 30th passage. NOCC cells secreted 750.5 ng/ml of VEGF over 3 days of culture. Hypoxia inducible factor-1α (HIF-1α) is a primary regulator of VEGF under hypoxic conditions. NOCC cells were not sensitive to the anticancer drugs BEV, DOX, GEM, ETP, CDDP, or TXT. The graft of NOCC cells to a scid mouse displayed similar histological aspects to the original tumor. Both the NOCC cells and the graft of the NOCC cells gave a positive PAS reaction.