New technique for mass rearing Bradysia odoriphaga
New technique for mass rearing Bradysia odoriphaga
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DOI：
10.7679/j.issn.2095-1353.2016.057
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发表时间：
2016-03-01
期刊：
影响因子：
--
通讯作者：
Yu Yi
中科院分区：
文献类型：
--
作者：
Chen Hao;Feng Lian-Jie;Yu Yi
【目的】 韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang是为害韭菜等蔬菜的重要害虫，规模化饲养提供大量虫源是其他研究工作得以开展的基础。本研究针对现有饲养技术存在的饲养成本高、饲养动物数量少、饲养材料和人工成本高等问题，提出了一种新的饲养技术--人工饲养法。[方法]实验饲养B.结果表明，在马铃薯、花生和豆类上，韭菜瘿蚊幼虫的存活率、产卵量、羽化节律和性比均无显著性差异。与韭菜和人工饲料饲养的食虫相比，韭菜和人工饲料饲养的食虫量增加。[结果] B的成活率和繁殖力无显著差异。韭菜和人工饲料饲养的幼虫。用新饲料饲养的幼虫，从卵到成虫的发育历期为20-23 d，性比为0.8：1 ~ 1.21。虽然新饲料易感染真菌，但分子鉴定结果显示，感染的主要真菌种类为秀丽放线毛霉、卷曲背枯菌、黄曲霉和赭曲霉，均为腐生菌，不影响B.恐蚊【目的】 韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang是为害韭菜等蔬菜的重要害虫，规模化饲养提供大量虫源是其他研究工作得以开展的基础。
[Objectives] Bradysia odoriphaga Yang et Zhang is an important pest of Chinese chives. Large scale rearing of this pest is useful for developing methods to control it. Our study addressed the high material and labor costs, and relatively small number of animals produced, by the present rearing technique. [Methods] We experimentally reared B. odoriphaga larvae on potatoes, peanuts and beans and compared the survival rate, fecundity, emergence rhythm and sex ratio of B. odoriphaga reared on this diet to those reared on Chinese chives and artificial diets. [Results] There was no significant difference between the survival rate and fecundity of B. odoriphaga larvae reared on the new diet and those reared on Chinese chives and artificial diets. The developmental period from egg to adult of larvae reared on the new diet was 20-23 days and the sex ratio was 0.8 : 1-1.2 1. Although the new diet became easily infected with fungi, molecular identification revealed that the main fungal species were Actinomucor elegans, Backusella circina, Aspergillus flavus and Aspergillus ochraceus, all of which are saprophytic and did not affect the survival and breeding of B. odoriphaga. [Conclusion] Our technique saved time, labor and materials, and is especially suitable for the maintenance of laboratory population and mass rearing.