Polymorphism of the MICA gene and risk for oral submucous fibrosis
Polymorphism of the MICA gene and risk for oral submucous fibrosis
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DOI：
10.1111/j.1600-0714.2004.00047.x
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Dang, CW
中科院分区：
文献类型：
--
作者：
Liu, CJ;Lee, YJ;Dang, CW
背景技术背景：口腔粘膜下纤维性变（OSF）是一种潜在的、癌前的慢性疾病，可能影响整个口腔，有时会延伸到咽部，据报道与免疫功能有关。主要组织相容性复合体（MHC）I类链相关基因A（云母）由角质形成细胞和其他上皮细胞表达，其编码的蛋白质与位于粘膜下层的γ/δ T细胞相互作用。云母基因在跨膜区有一个三重重复序列（GCT）多态性，导致5种不同的等位基因模式。方法：采用ABI Prism 377-18 DNA测序仪分析80例OSF患者和351例随机选择的无关对照的云母多态性。用Genescan 672软件估计微卫星重复序列的数目结果：OSF患者云母基因A6等位基因的表型频率显著高于对照组（OR = 3.48，95%CI = 1.8-6.71，P = 0.0002），等位基因频率（OR = 2.65，95%CI = 1.44-4.86，P = 0.001）。
BACKGROUND: Oral submucous fibrosis (OSF), an insidious, pre-cancerous, chronic disease that may affect the entire oral cavity and sometimes extend to the pharynx has been reported to be associated with immune function. The major histocompatibility complex (MHC) class I chain-related gene A (MICA) is expressed by keratinocytes and other epithelial cells, and its encoded protein interacts with gamma/delta T-cells localized in the submucosa. The MICA gene has a triplet repeat (GCT) polymorphism in the transmembrane domain resulting in five distinct allelic patterns.METHODS: We analyzed MICA polymorphism in 80 OSF patients and 351 randomly selected unrelated controls by using the ABI Prism 377-18 DNA sequencer (Applied Biosystems) to analyze the sample DNA PCR products. The number of microsatellite repeats was estimated with Genescan 672 software (Applied Biosystems) with a standard size marker of GS-350 TAMRA.RESULTS: The phenotype frequency of allele A6 of MICA in subjects with OSF was significantly higher than that in controls (OR = 3.48; 95% CI = 1.8-6.71; P = 0.0002), as was the frequency of the allele (OR = 2.65; 95% CI = 1.44-4.86; P = 0.001).CONCLUSION: The results suggest that allele A6 in MICA might confer a risk for OSF.